| Autor: |
Jochems PGM; Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands., Keusters WR; Julius Centre, University Medical Center Utrecht, Utrecht, The Netherlands., America AHP; Wageningen Plant Research, Wageningen University, Wageningen, The Netherlands., Rietveld PCS; Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands., Bastiaan-Net S; Wageningen Food & Biobased Research, Wageningen University, Wageningen, The Netherlands., Ariëns RMC; Wageningen Food & Biobased Research, Wageningen University, Wageningen, The Netherlands., Tomassen MMM; Wageningen Food & Biobased Research, Wageningen University, Wageningen, The Netherlands., Lewis F; Danone Nutricia Research, Utrecht, The Netherlands., Li Y; Department of Orthopaedics, University Medical Center Utrecht, Utrecht, The Netherlands., Westphal KGC; Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands., Garssen J; Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.; Danone Nutricia Research, Utrecht, The Netherlands., Wichers HJ; Wageningen Food & Biobased Research, Wageningen University, Wageningen, The Netherlands., van Bergenhenegouwen J; Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.; Danone Nutricia Research, Utrecht, The Netherlands., Masereeuw R; Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands. r.masereeuw@uu.nl. |
| Abstrakt: |
Food security is under increased pressure due to the ever-growing world population. To tackle this, alternative protein sources need to be evaluated for nutritional value, which requires information on digesta peptide composition in comparison to established protein sources and coupling to biological parameters. Here, a combined experimental and computational approach is presented, which compared seventeen protein sources with cow's whey protein concentrate (WPC) as the benchmark. In vitro digestion of proteins was followed by proteomics analysis and statistical model-based clustering. Information on digesta peptide composition resulted in 3 cluster groups, primarily driven by the peptide overlap with the benchmark protein WPC. Functional protein data was then incorporated in the computational model after evaluating the effects of eighteen protein digests on intestinal barrier integrity, viability, brush border enzyme activity, and immune parameters using a bioengineered intestine as microphysiological gut system. This resulted in 6 cluster groups. Biological clustering was driven by viability, brush border enzyme activity, and significant differences in immune parameters. Finally, a combination of proteomic and biological efficacy data resulted in 5 clusters groups, driven by a combination of digesta peptide composition and biological effects. The key finding of our holistic approach is that protein source (animal, plant or alternative derived) is not a driving force behind the delivery of bioactive peptides and their biological efficacy. |
