Estrous Cycle Modulation of Feeding and Relaxin-3/Rxfp3 mRNA Expression: Implications for Estradiol Action.

Autor:	de Ávila C; Department of Psychiatry and Neuroscience, Faculty of Medicine, CRIUCPQ, Université Laval, Québec, Québec, Canada, Avila.Camila@mayo.edu.; Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark, Avila.Camila@mayo.edu.; Department of Neuroscience, Mayo Clinic, Scottsdale, Arizona, USA, Avila.Camila@mayo.edu.; Department of Psychology, Arizona State University, Tempe, Arizona, USA, Avila.Camila@mayo.edu., Chometton S; Department of Psychiatry and Neuroscience, Faculty of Medicine, CRIUCPQ, Université Laval, Québec, Québec, Canada., Calvez J; Department of Psychiatry and Neuroscience, Faculty of Medicine, CRIUCPQ, Université Laval, Québec, Québec, Canada., Guèvremont G; Department of Psychiatry and Neuroscience, Faculty of Medicine, CRIUCPQ, Université Laval, Québec, Québec, Canada., Kania A; Department of Neurophysiology and Chronobiology, Institute of Zoology and Biomedical Research, Jagiellonian University, Krakow, Poland., Torz L; Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark.; NNF CBMR, Nutrient and Metabolite Sensing, Faculty of Health and Medical Science, University of Copenhagen, Copenhagen, Denmark., Lenglos C; Department of Psychiatry and Neuroscience, Faculty of Medicine, CRIUCPQ, Université Laval, Québec, Québec, Canada., Blasiak A; Department of Neurophysiology and Chronobiology, Institute of Zoology and Biomedical Research, Jagiellonian University, Krakow, Poland., Rosenkilde MM; Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark., Holst B; Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark.; NNF CBMR, Nutrient and Metabolite Sensing, Faculty of Health and Medical Science, University of Copenhagen, Copenhagen, Denmark., Conrad CD; Department of Psychology, Arizona State University, Tempe, Arizona, USA., Fryer JD; Department of Neuroscience, Mayo Clinic, Scottsdale, Arizona, USA., Timofeeva E; Department of Psychiatry and Neuroscience, Faculty of Medicine, CRIUCPQ, Université Laval, Québec, Québec, Canada., Gundlach AL; The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Melbourne, Victoria, Australia., Cifani C; Department of Psychiatry and Neuroscience, Faculty of Medicine, CRIUCPQ, Université Laval, Québec, Québec, Canada.; School of Pharmacy, Pharmacology Unit, University of Camerino, Camerino, Italy.
Jazyk:	angličtina
Zdroj:	Neuroendocrinology [Neuroendocrinology] 2021; Vol. 111 (12), pp. 1201-1218. Date of Electronic Publication: 2020 Dec 17.
DOI:	10.1159/000513830
Abstrakt:	Introduction: Food intake varies during the ovarian hormone/estrous cycle in humans and rodents, an effect mediated mainly by estradiol. A potential mediator of the central anorectic effects of estradiol is the neuropeptide relaxin-3 (RLN3) synthetized in the nucleus incertus (NI) and acting via the relaxin family peptide-3 receptor (RXFP3). Methods: We investigated the relationship between RLN3/RXFP3 signaling and feeding behavior across the female rat estrous cycle. We used in situ hybridization to investigate expression patterns of Rln3 mRNA in NI and Rxfp3 mRNA in the hypothalamic paraventricular nucleus (PVN), lateral hypothalamic area (LHA), medial preoptic area (MPA), and bed nucleus of the stria terminalis (BNST), across the estrous cycle. We identified expression of estrogen receptors (ERs) in the NI using droplet digital PCR and assessed the electrophysiological responsiveness of NI neurons to estradiol in brain slices. Results: Rln3 mRNA reached the lowest levels in the NI pars compacta during proestrus. Rxfp3 mRNA levels varied across the estrous cycle in a region-specific manner, with changes observed in the perifornical LHA, magnocellular PVN, dorsal BNST, and MPA, but not in the parvocellular PVN or lateral LHA. G protein-coupled estrogen receptor 1 (Gper1) mRNA was the most abundant ER transcript in the NI. Estradiol inhibited 33% of type 1 NI neurons, including RLN3-positive cells. Conclusion: These findings demonstrate that the RLN3/RXFP3 system is modulated by the estrous cycle, and although further studies are required to better elucidate the cellular and molecular mechanisms of estradiol signaling, current results implicate the involvement of the RLN3/RXFP3 system in food intake fluctuations observed across the estrous cycle in female rats. (© 2020 S. Karger AG, Basel.)
Databáze:	MEDLINE
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