| Autor: |
Victor EC; Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil., Goulardins J; Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil., Cardoso VO; Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil., Silva REC; Biomedicine undergraduate course, Universidade Nove de Julho (UNINOVE), Sao Paulo, Brazil., Brugnera A; Biomedical Engineer Research Center (CEB), Universidade Camilo Castelo Branco, São José dos Campos, Brazil., Bussadori SK; Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil.; Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil., Fernandes KPS; Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil., Mesquita-Ferrari RA; Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil.; Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho (UNINOVE), São Paulo, Brazil. |
| Abstrakt: |
Objective: To evaluate the effect of photobiomodulation (PBM) on cell viability, synthesis of nitric oxide (NO), and interleukin (IL)-6 inflammatory cytokine production in myoblasts cultured in the presence of lipopolysaccharides (LPSs). Methods: C2C12 myoblasts were treated with LPS and PBM using different parameters (wavelength: 780 nm; beam spot: 0.04 cm 2 ; power output: 10 or 40 mW; energy density: 5 or 20 J/cm 2 ; and 20-sec exposure time). Nonirradiated cells were used to the control group. Results: An increase in cell viability was found in both LPS groups in comparison with the control. PBM with the higher power output (40 mW) induced a reduction in cell viability. PBM also modulated the synthesis of NO in the myoblasts, but did not alter the expression of IL-6. Conclusions: Based on these findings, PBM is capable of modulating the cell viability and the production of NO in LPS-treated myoblasts and it is, therefore, a possible tool for the treatment of muscle injury caused by infection. |
