Selective activation of FZD7 promotes mesendodermal differentiation of human pluripotent stem cells.

Autor:	Gumber D; Department of Cellular & Molecular Medicine, University of California San Diego, San Diego, United States., Do M; Department of Cellular & Molecular Medicine, University of California San Diego, San Diego, United States., Suresh Kumar N; Department of Cellular & Molecular Medicine, University of California San Diego, San Diego, United States., Sonavane PR; Department of Cellular & Molecular Medicine, University of California San Diego, San Diego, United States., Wu CCN; Department of Medicine, University of California San Diego, San Diego, United States., Cruz LS; Department of Biology, San Diego State University, San Diego, United States., Grainger S; Department of Biology, San Diego State University, San Diego, United States., Carson D; Department of Medicine, University of California San Diego, San Diego, United States., Gaasterland T; University of California San Diego and Scripps Institution of Oceanography, Scripps Genome Center, La Jolla, United States., Willert K; Department of Cellular & Molecular Medicine, University of California San Diego, San Diego, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2020 Dec 17; Vol. 9. Date of Electronic Publication: 2020 Dec 17.
DOI:	10.7554/eLife.63060
Abstrakt:	WNT proteins are secreted symmetry breaking signals that interact with cell surface receptors of the FZD family to regulate a multitude of developmental processes. Studying selectivity between WNTs and FZDs has been hampered by the paucity of purified WNT proteins and by their apparent non-selective interactions with the FZD receptors. Here, we describe an engineered protein, called F7L6, comprised of antibody-derived single-chain variable fragments, that selectively binds to human FZD7 and the co-receptor LRP6. F7L6 potently activates WNT/β-catenin signaling in a manner similar to Wnt3a. In contrast to Wnt3a, F7L6 engages only FZD7 and none of the other FZD proteins. Treatment of human pluripotent stem (hPS) cells with F7L6 initiates transcriptional programs similar to those observed during primitive streak formation and subsequent gastrulation in the mammalian embryo. This demonstrates that selective engagement and activation of FZD7 signaling is sufficient to promote mesendodermal differentiation of hPS cells. Competing Interests: DG, MD, NS, PS, CW, LC, SG, DC, TG, KW No competing interests declared (© 2020, Gumber et al.)
Databáze:	MEDLINE
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