Further insights into the eco-epidemiology of American cutaneous leishmaniasis in the Belem metropolitan region, Pará State, Brazil.

Autor:	Gonçalves LP; Instituto Evandro Chagas, Laboratório de Leishmanioses 'Prof. Dr. Ralph Lainson', Seção de Parasitologia, Ananindeua, PA, Brasil., Santos TVD; Instituto Evandro Chagas, Laboratório de Leishmanioses 'Prof. Dr. Ralph Lainson', Seção de Parasitologia, Ananindeua, PA, Brasil., Campos MB; Instituto Evandro Chagas, Laboratório de Leishmanioses 'Prof. Dr. Ralph Lainson', Seção de Parasitologia, Ananindeua, PA, Brasil., Lima LVDR; Instituto Evandro Chagas, Laboratório de Leishmanioses 'Prof. Dr. Ralph Lainson', Seção de Parasitologia, Ananindeua, PA, Brasil., Ishikawa EAY; Universidade Federal do Pará, Núcleo de Medicina Tropical, Belém, PA, Brasil., Silveira FT; Instituto Evandro Chagas, Laboratório de Leishmanioses 'Prof. Dr. Ralph Lainson', Seção de Parasitologia, Ananindeua, PA, Brasil.; Universidade Federal do Pará, Núcleo de Medicina Tropical, Belém, PA, Brasil., Ramos PKS; Instituto Evandro Chagas, Laboratório de Leishmanioses 'Prof. Dr. Ralph Lainson', Seção de Parasitologia, Ananindeua, PA, Brasil.
Jazyk:	angličtina
Zdroj:	Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2020 Dec 11; Vol. 53, pp. e20200255. Date of Electronic Publication: 2020 Dec 11 (Print Publication: 2020).
DOI:	10.1590/0037-8682-0255-2020
Abstrakt:	Introduction: In the Belém Metropolitan Region (BMR), Pará State, Brazil, American cutaneous leishmaniasis (ACL) is endemic; however, very little is known regarding its causative agents. Therefore, we used our standard diagnostic approach combined with an RNA polymerase II largest subunit (RNAPOIILS)-polymerase chain reaction (PCR) followed by analysis of restriction fragment length polymorphism (PCR-RFLP) to identify Leishmania spp. ACL agents in this region. Methods: Thirty-two Leishmania spp. isolates from patients with ACL in the BMR during 1995-2018 were analyzed. Leishmania spp. DNA samples were amplified using the primers RPOR2/RPOF2, and the 615-bp PCR products were subjected to enzymatic digestion using TspRI and HgaI endonucleases. Results: ACL etiological agents in the BMR comprised Leishmania (Viannia) lindenbergi (43.7%) followed by Leishmania (Viannia) lainsoni (34.4%), Leishmania (Leishmania) amazonensis (12.5%), and Leishmania (Viannia) braziliensis (9.4%). Conclusions: To our knowledge, the results of the study revealed for the first time that L. (V.) lindenbergi and L. (V.) lainsoni are the main ACL agents in BMR.
Databáze:	MEDLINE
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