Integrative characterization of the near-minimal bacterium Mesoplasma florum.

Autor: Matteau D; Département de biologie, Université de Sherbrooke, Sherbrooke, QC, Canada., Lachance JC; Département de biologie, Université de Sherbrooke, Sherbrooke, QC, Canada., Grenier F; Département de biologie, Université de Sherbrooke, Sherbrooke, QC, Canada., Gauthier S; Département de biologie, Université de Sherbrooke, Sherbrooke, QC, Canada., Daubenspeck JM; Department of Genetics, University of Alabama at Birmingham, Birmingham, AL, USA., Dybvig K; Department of Genetics, University of Alabama at Birmingham, Birmingham, AL, USA., Garneau D; Département de biologie, Université de Sherbrooke, Sherbrooke, QC, Canada., Knight TF; Ginkgo Bioworks, Boston, MA, USA., Jacques PÉ; Département de biologie, Université de Sherbrooke, Sherbrooke, QC, Canada., Rodrigue S; Département de biologie, Université de Sherbrooke, Sherbrooke, QC, Canada.
Jazyk: angličtina
Zdroj: Molecular systems biology [Mol Syst Biol] 2020 Dec; Vol. 16 (12), pp. e9844.
DOI: 10.15252/msb.20209844
Abstrakt: The near-minimal bacterium Mesoplasma florum is an interesting model for synthetic genomics and systems biology due to its small genome (~ 800 kb), fast growth rate, and lack of pathogenic potential. However, fundamental aspects of its biology remain largely unexplored. Here, we report a broad yet remarkably detailed characterization of M. florum by combining a wide variety of experimental approaches. We investigated several physical and physiological parameters of this bacterium, including cell size, growth kinetics, and biomass composition of the cell. We also performed the first genome-wide analysis of its transcriptome and proteome, notably revealing a conserved promoter motif, the organization of transcription units, and the transcription and protein expression levels of all protein-coding sequences. We converted gene transcription and expression levels into absolute molecular abundances using biomass quantification results, generating an unprecedented view of the M. florum cellular composition and functions. These characterization efforts provide a strong experimental foundation for the development of a genome-scale model for M. florum and will guide future genome engineering endeavors in this simple organism.
(© 2020 The Authors. Published under the terms of the CC BY 4.0 license.)
Databáze: MEDLINE
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