Chemoprevention of DMH-Induced Early Colon Carcinogenesis in Male BALB/c Mice by Administration of Lactobacillus Paracasei DTA81.

Autor:	da Silva Duarte V; Department of Agronomy Food Natural Resources Animals and Environment, University of Padova, Viale dell'Universitá, 16, 35020 Legnaro (PD), Italy.; Department of Microbiology, Av. Peter Henry Rolfs, s/n, Campus Universitário, Universidade Federal de Viçosa, 36570-900 Vicosa, Brazil., Dos Santos Cruz BC; Department of Nutrition and Health, Av. Peter Henry Rolfs, s/n, Campus Universitário, Universidade Federal de Vicosa, 36570-900 Vicosa, Brazil., Tarrah A; Department of Agronomy Food Natural Resources Animals and Environment, University of Padova, Viale dell'Universitá, 16, 35020 Legnaro (PD), Italy., Sousa Dias R; Department of General Biology, Av. Peter Henry Rolfs, s/n, Campus Universitario, Universidade Federal de Vicosa, 36570-900 Vicosa, Brazil., de Paula Dias Moreira L; Department of Agronomy Food Natural Resources Animals and Environment, University of Padova, Viale dell'Universitá, 16, 35020 Legnaro (PD), Italy., Lemos Junior WJF; Faculty of Science and Technology, Free University of Bolzano-Bozen, 39100 Bolzano, Italy., Fidélis Silva LC; Department of Microbiology, Av. Peter Henry Rolfs, s/n, Campus Universitário, Universidade Federal de Viçosa, 36570-900 Vicosa, Brazil., Rocha Santana G; Department of General Biology, Av. Peter Henry Rolfs, s/n, Campus Universitario, Universidade Federal de Vicosa, 36570-900 Vicosa, Brazil., Licursi de Oliveira L; Department of General Biology, Av. Peter Henry Rolfs, s/n, Campus Universitario, Universidade Federal de Vicosa, 36570-900 Vicosa, Brazil., Gouveia Peluzio MDC; Department of Nutrition and Health, Av. Peter Henry Rolfs, s/n, Campus Universitário, Universidade Federal de Vicosa, 36570-900 Vicosa, Brazil., Mantovani HC; Department of Microbiology, Av. Peter Henry Rolfs, s/n, Campus Universitário, Universidade Federal de Viçosa, 36570-900 Vicosa, Brazil., Corich V; Department of Agronomy Food Natural Resources Animals and Environment, University of Padova, Viale dell'Universitá, 16, 35020 Legnaro (PD), Italy., Giacomini A; Department of Agronomy Food Natural Resources Animals and Environment, University of Padova, Viale dell'Universitá, 16, 35020 Legnaro (PD), Italy., de Paula SO; Department of General Biology, Av. Peter Henry Rolfs, s/n, Campus Universitario, Universidade Federal de Vicosa, 36570-900 Vicosa, Brazil.
Jazyk:	angličtina
Zdroj:	Microorganisms [Microorganisms] 2020 Dec 14; Vol. 8 (12). Date of Electronic Publication: 2020 Dec 14.
DOI:	10.3390/microorganisms8121994
Abstrakt:	We evaluated the effects of the probiotic candidate Lactobacillus paracasei DTA81 (DTA81) on liver oxidative stress, colonic cytokine profile, and gut microbiota in mice with induced early colon carcinogenesis (CRC) by 1,2-dimethylhydrazine (DMH). Animals were divided into four different groups ( n = 6) and received the following treatments via orogastric gavage for 8 weeks: Group skim milk (GSM): 300 mg/freeze-dried skim milk/day; Group L. paracasei DTA81 (DTA81): 3 × 10 9 colony-forming units (CFU)/day; Group Lactobacillus rhamnosus GG (LGG): 3 × 10 9 CFU/day; Group non-intervention (GNI): 0.1 mL/water/day. A single DMH dose (20 mg/kg body weight) was injected intraperitoneally (i.p), weekly, in all animals (seven applications in total). At the end of the experimental period, DTA81 intake reduced hepatic levels of carbonyl protein and malondialdehyde (MDA). Moreover, low levels of the pro-inflammatory cytokines Interleukin-6 (IL-6) and IL-17, as well as a reduced expression level of the proliferating cell nuclear antigen (PCNA) were observed in colonic homogenates. Lastly, animals who received DTA81 showed an intestinal enrichment of the genus Ruminiclostridium and increased concentrations of caecal acetic acid and total short-chain fatty acids. In conclusion, this study indicates that the administration of the probiotic candidate DTA81 can have beneficial effects on the initial stages of CRC development.
Databáze:	MEDLINE
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