Broiler Farms and Carcasses Are an Important Reservoir of Multi-Drug Resistant Escherichia coli in Ecuador.

Autor: Ortega-Paredes D; Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencia a los Antimicrobianos (UNIETAR), Facultad de Medicina Veterinaria y Zootecnia, Universidad Central del Ecuador, Quito, Ecuador., de Janon S; Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencia a los Antimicrobianos (UNIETAR), Facultad de Medicina Veterinaria y Zootecnia, Universidad Central del Ecuador, Quito, Ecuador., Villavicencio F; Centro de Referencia Nacional de Resistencia a los Antimicrobianos, Instituto Nacional de Investigación en Salud Pública 'Leopoldo Izquieta Pérez', Quito, Ecuador., Ruales KJ; Centro de Referencia Nacional de Resistencia a los Antimicrobianos, Instituto Nacional de Investigación en Salud Pública 'Leopoldo Izquieta Pérez', Quito, Ecuador., De La Torre K; Facultad de Medicina, Pontificia Universidad Católica del Ecuador, Quito, Ecuador., Villacís JE; Centro de Referencia Nacional de Resistencia a los Antimicrobianos, Instituto Nacional de Investigación en Salud Pública 'Leopoldo Izquieta Pérez', Quito, Ecuador.; Facultad de Medicina, Pontificia Universidad Católica del Ecuador, Quito, Ecuador., Wagenaar JA; Wageningen Bioveterinary Research, Lelystad, Netherlands.; Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands., Matheu J; Department of Food Safety and Zoonoses, World Health Organization, Geneva, Switzerland., Bravo-Vallejo C; Hospital General del Sur Quito-Instituto Ecuatoriano de Seguridad Social (IESS), Quito, Ecuador., Fernández-Moreira E; Carrera de Medicina, Facultad de Ciencias de la Salud, Universidad de las Américas, Quito, Ecuador., Vinueza-Burgos C; Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencia a los Antimicrobianos (UNIETAR), Facultad de Medicina Veterinaria y Zootecnia, Universidad Central del Ecuador, Quito, Ecuador.
Jazyk: angličtina
Zdroj: Frontiers in veterinary science [Front Vet Sci] 2020 Nov 25; Vol. 7, pp. 547843. Date of Electronic Publication: 2020 Nov 25 (Print Publication: 2020).
DOI: 10.3389/fvets.2020.547843
Abstrakt: Antimicrobial resistance (AMR) is a major health threat for public and animal health in the twenty-first century. In Ecuador, antibiotics have been used by the poultry industry for decades resulting in the presence of multi-drug resistant (MDR) bacteria in the poultry meat production chain, with the consequent risk for public health. This study evaluated the prevalence of ESBL/AmpC and mcr genes in third-generation cephalosporin-resistant Escherichia coli (3GC-R E. coli ) isolated from broiler farms (animal component), broiler carcasses (food component), and human enteritis (human component) in Quito-Ecuador. Samples were collected weekly from November 2017 to November 2018. For the animal, food, and human components, 133, 335, and 302 samples were analyzed, respectively. Profiles of antimicrobial resistance were analyzed by an automated microdilution system. Resistance genes were studied by PCR and Sanger sequencing. From all samples, 122 (91.7%), 258 (77%), and 146 (48.3%) samples were positive for 3GC-R E. coli in the animal, food, and human components, respectively. Most of the isolates (472/526, 89.7%) presented MDR phenotypes. The ESBL bla CTX- M- 55 , bla CTX- M- 3 , bla CTX- M- 15 , bla CTX- M- 65 , bla CTX- M- 27 , and bla CTX- M- 14 were the most prevalent ESBL genes while bla CMY-2 was the only AmpC detected gene. The mcr -1 gene was found in 20 (16.4%), 26 (10.1%), and 3 (2.1%) of isolates from animal, food, and human components, respectively. The implication of poultry products in the prevalence of ESBL/AmpC and mcr genes in 3GC-R must be considered in the surveillance of antimicrobial resistance.
(Copyright © 2020 Ortega-Paredes, de Janon, Villavicencio, Ruales, De La Torre, Villacís, Wagenaar, Matheu, Bravo-Vallejo, Fernández-Moreira and Vinueza-Burgos.)
Databáze: MEDLINE
Externí odkaz: