ELISA Test for the Serological Detection of Scedosporium / Lomentospora in Cystic Fibrosis Patients.
| Autor: | Martin-Souto L; Fungal and Bacterial Biomics Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, Spain., Buldain I; Fungal and Bacterial Biomics Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, Spain., Areitio M; Fungal and Bacterial Biomics Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, Spain., Aparicio-Fernandez L; Fungal and Bacterial Biomics Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, Spain., Antoran A; Fungal and Bacterial Biomics Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, Spain., Bouchara JP; Groupe d'Etude des Interactions Hôte-Pathogène (EA 3142), SFR ICAT 4208, Institut de Biologie en Santé-IRIS, Centre Hospitalier Universitaire, Angers, France., Martin-Gomez MT; Microbiology Department, Vall d'Hebron University Hospital, Barcelona, Spain., Rementeria A; Fungal and Bacterial Biomics Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, Spain., Hernando FL; Fungal and Bacterial Biomics Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, Spain., Ramirez-Garcia A; Fungal and Bacterial Biomics Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, Spain. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2020 Nov 26; Vol. 10, pp. 602089. Date of Electronic Publication: 2020 Nov 26 (Print Publication: 2020). |
| DOI: | 10.3389/fcimb.2020.602089 |
| Abstrakt: | The detection and diagnosis of the opportunistic fungi Scedosporium spp. and Lomentospora prolificans still relies mainly on low-sensitive culture-based methods. This fact is especially worrying in Cystic Fibrosis (CF) patients in whom these fungal species are frequently isolated and may increase the risk of suffering from an infection or other health problems. Therefore, with the purpose of developing a serologic detection method for Scedosporium / Lomentospora , four different Scedosporium boydii protein extracts (whole cell protein extract, secretome, total cell surface and conidial surface associated proteins) were studied by ELISA to select the most useful for IgG detection in sera from CF patients. The four extracts were able to discriminate the Scedosporium / Lomentospora -infected from Aspergillus- infected and non-infected patients. However, the whole cell protein extract was the one selected, as it was the one with the highest output in terms of protein concentration per ml of fungal culture used, and its discriminatory capacity was the best. The ELISA test developed was then assayed with 212 sera from CF patients and it showed to be able to detect Scedosporium spp. and Lomentospora prolificans with very high sensitivity and specificity, 86%-100% and 93%-99%, respectively, depending on the cut-off value chosen (four values were proposed A Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2020 Martin-Souto, Buldain, Areitio, Aparicio-Fernandez, Antoran, Bouchara, Martin-Gomez, Rementeria, Hernando and Ramirez-Garcia.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|