The mRNA Distribution of Cancer Stem Cell Marker CD90/Thy-1 Is Comparable in Hepatocellular Carcinoma of Eastern and Western Populations.

Autor: Luong AB; Fondazione Italiana Fegato ONLUS, AREA Science Park Basovizza, 34149 Trieste, Italy.; Center for Molecular Biomedicine, University of Medicine and Pharmacy at Ho Chi Minh, Ho Chi Minh City 700000, Vietnam., Do HQ; Fondazione Italiana Fegato ONLUS, AREA Science Park Basovizza, 34149 Trieste, Italy.; Laboratory of Stem Cell Research and Application, VNUHCM-University of Science, Ho Chi Minh City 700000, Vietnam., Tarchi P; Clinical Surgery Unit, Azienda Sanitaria Universitaria Giuliana Isontina (ASUGI), 34148 Trieste, Italy., Bonazza D; Surgical Pathology Unit, Azienda Sanitaria Universitaria Giuliana Isontina (ASUGI), 34148 Trieste, Italy., Bottin C; Department of Medical, Surgical and Health Sciences, University of Trieste, 34127 Trieste, Italy., Cabral LKD; Fondazione Italiana Fegato ONLUS, AREA Science Park Basovizza, 34149 Trieste, Italy.; Doctoral School in Molecular Biomedicine, University of Trieste, 34127 Trieste, Italy., Tran LDC; University Medical Center, University of Medicine and Pharmacy at Ho Chi Minh, Ho Chi Minh City 700000, Vietnam., Doan TPT; Department of Pathology, University of Medicine and Pharmacy at Ho Chi Minh, Ho Chi Minh City 700000, Vietnam., Crocè LS; Fondazione Italiana Fegato ONLUS, AREA Science Park Basovizza, 34149 Trieste, Italy.; Department of Medical, Surgical and Health Sciences, University of Trieste, 34127 Trieste, Italy., Pham HLT; University Medical Center, University of Medicine and Pharmacy at Ho Chi Minh, Ho Chi Minh City 700000, Vietnam., Tiribelli C; Fondazione Italiana Fegato ONLUS, AREA Science Park Basovizza, 34149 Trieste, Italy., Sukowati CHC; Fondazione Italiana Fegato ONLUS, AREA Science Park Basovizza, 34149 Trieste, Italy.
Jazyk: angličtina
Zdroj: Cells [Cells] 2020 Dec 12; Vol. 9 (12). Date of Electronic Publication: 2020 Dec 12.
DOI: 10.3390/cells9122672
Abstrakt: Epidemiology of hepatocellular carcinoma (HCC) showed a correlation between incidence and geographical-relevant risk factors. This study aims to compare the distributions of cancer stem cells (CSC) in two distant populations in Asia and Europe. We analyzed 52 and 43 selected HCC patients undergoing hepatectomy in Ho Chi Minh City (Vietnam) and Trieste (Italy). Each patient sample consisted of HCC, peri-HCC, and non-tumoral (distal) tissue. Demographic data were recorded together with clinical findings. The protocol for the collection of tissue samples and RNA was standardized in both laboratories and gene expression analysis was performed in a single laboratory with identical PCR conditions. Baseline data showed comparable laboratory findings between the two cohorts. mRNA distribution showed a comparable pattern of all CSC markers analyzed with the expression of CD90 progressively increasing from distal and peri-HCC to be highest in HCC ( p < 0.001), confirmed by immunofluorescence data. CD90 mRNA distribution was related to HBV-related HCC and a tumor diameter less than 5 cm. Patients with high tumoral CD90 mRNA had a shorter time ( p < 0.05) to tumor recurrence compared to patients with lower CD90. This comparative study showed that CD90 mRNA expressions are comparable between Eastern and Western HCC cases.
Databáze: MEDLINE
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