| Autor: |
Fonseca DFS; CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal., Carvalho JPF; CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal., Bastos V; Department of Biology and CESAM, University of Aveiro, 3810-193 Aveiro, Portugal., Oliveira H; Department of Biology and CESAM, University of Aveiro, 3810-193 Aveiro, Portugal., Moreirinha C; CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal., Almeida A; Department of Biology and CESAM, University of Aveiro, 3810-193 Aveiro, Portugal., Silvestre AJD; CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal., Vilela C; CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal., Freire CSR; CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal. |
| Abstrakt: |
Antibacterial multi-layered patches composed of an oxidized bacterial cellulose (OBC) membrane loaded with dexpanthenol (DEX) and coated with several chitosan (CH) and alginate (ALG) layers were fabricated by spin-assisted layer-by-layer (LbL) assembly. Four patches with a distinct number of layers (5, 11, 17, and 21) were prepared. These nanostructured multi-layered patches reveal a thermal stability up to 200 °C, high mechanical performance (Young's modulus ≥ 4 GPa), and good moisture-uptake capacity (240-250%). Moreover, they inhibited the growth of the skin pathogen Staphylococcus aureus (3.2-log CFU mL -1 reduction) and were non-cytotoxic to human keratinocytes (HaCaT cells). The in vitro release profile of DEX was prolonged with the increasing number of layers, and the time-dependent data imply a diffusion/swelling-controlled drug release mechanism. In addition, the in vitro wound healing assay demonstrated a good cell migration capacity, headed to a complete gap closure after 24 h. These results certify the potential of these multi-layered polysaccharides-based patches toward their application in wound healing. |
