| Autor: |
Silva VC; Laboratory of Pharmaceutical Technology, Research Program Post-Graduation in Pharmaceutical Sciences, Institute of Pharmaceutical Sciences, Federal University of Alagoas (UFAL), Maceió, Alagoas 57072-970, Brazil.; Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, Alagoas 57072-970, Brazil., Silva AMGS; Laboratory of Pharmaceutical Technology, Research Program Post-Graduation in Pharmaceutical Sciences, Institute of Pharmaceutical Sciences, Federal University of Alagoas (UFAL), Maceió, Alagoas 57072-970, Brazil., Basílio JAD; Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, Alagoas 57072-970, Brazil., Xavier JA; Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, Alagoas 57072-970, Brazil., do Nascimento TG; Laboratory of Pharmaceutical Technology, Research Program Post-Graduation in Pharmaceutical Sciences, Institute of Pharmaceutical Sciences, Federal University of Alagoas (UFAL), Maceió, Alagoas 57072-970, Brazil., Naal RMZG; Department of BioMolecular Sciences, Faculty of Pharmaceutical Sciences of Ribeirão Preto. Avenida do Café, s/n, Ribeirão Preto, São Paulo 14040-903, Brazil., Del Lama MP; Department of BioMolecular Sciences, Faculty of Pharmaceutical Sciences of Ribeirão Preto. Avenida do Café, s/n, Ribeirão Preto, São Paulo 14040-903, Brazil., Leonelo LAD; Department of BioMolecular Sciences, Faculty of Pharmaceutical Sciences of Ribeirão Preto. Avenida do Café, s/n, Ribeirão Preto, São Paulo 14040-903, Brazil., Mergulhão NLON; Laboratory of Pharmaceutical Technology, Research Program Post-Graduation in Pharmaceutical Sciences, Institute of Pharmaceutical Sciences, Federal University of Alagoas (UFAL), Maceió, Alagoas 57072-970, Brazil.; Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, Alagoas 57072-970, Brazil., Maranhão FCA; Institute of Biological Science and Health, Federal University of Alagoas, Maceió, Alagoas 57072-970, Brazil., Silva DMW; Institute of Biological Science and Health, Federal University of Alagoas, Maceió, Alagoas 57072-970, Brazil., Owen R; Division of Preventive Oncology, German Cancer Research Center, Im Neuenheimer Feld 460, 69120 Heidelberg, Germany., Duarte IFB; Laboratory of Pharmaceutical Technology, Research Program Post-Graduation in Pharmaceutical Sciences, Institute of Pharmaceutical Sciences, Federal University of Alagoas (UFAL), Maceió, Alagoas 57072-970, Brazil.; Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, Alagoas 57072-970, Brazil., Bulhões LCG; Laboratory of Pharmaceutical Technology, Research Program Post-Graduation in Pharmaceutical Sciences, Institute of Pharmaceutical Sciences, Federal University of Alagoas (UFAL), Maceió, Alagoas 57072-970, Brazil.; Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, Alagoas 57072-970, Brazil., Basílio ID Jr; Laboratory of Pharmaceutical Technology, Research Program Post-Graduation in Pharmaceutical Sciences, Institute of Pharmaceutical Sciences, Federal University of Alagoas (UFAL), Maceió, Alagoas 57072-970, Brazil., Goulart MOF; Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, Alagoas 57072-970, Brazil. |
| Abstrakt: |
The main objectives of this study were to evaluate the chemical constitution and allergenic potential of red propolis extract (RPE). They were evaluated, using high performance liquid chromatography (HPLC) and the release of β-hexosaminidase, respectively. A plethora of biologically active polyphenols and the absence of allergic responses were evinced. RPE inhibited the release of β-hexosaminidase, suggesting that the extract does not stimulate allergic responses. Additionally, the physicochemical properties and antibacterial activity of hydrogel membranes loaded with RPE were analyzed. Bio-polymeric hydrogel membranes (M) were obtained using 5% carboxymethylcellulose (M1 and M2), 1.0% of citric acid (M3) and 10% RPE (for all). Their characterization was performed using thermal analysis, Fourier transform infrared (FTIR), total phenolic content, phenol release test and, antioxidant activity through 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and Ferric Reducing Antioxidant Power (FRAP). The latter appointed to the similar antioxidant capacity of the M1, M2 and M3. The degradation profiles showed higher thermostability to M3, followed by M2 and M1. The incorporation of RPE into the matrices and the crosslinking of M3 were evinced by FTIR. There were differences in the release of phenolic compounds, with a higher release related to M1 and lower in the strongly crosslinked M3. The degradation profiles showed higher thermostability to M3, followed by M2 and M1. The antibacterial activity of the membranes was determined using the disc diffusion assay, in comparison with controls, obtained in the same way, without RPE. The membranes elicited antibacterial activity against Staphylococcus aureus and Staphylococcus epidermidis , with superior performance over M3. The hydrogel membranes loaded with RPE promote a physical barrier against bacterial skin infections and may be applied in the wound healing process. |
