Characterizing the diverse cells that associate with the developing commissures of the zebrafish forebrain.

Autor:	Schnabl J; Department of Molecular and Cellular Biology, University of Massachusetts, Amherst, MA, USA., Litz MPH; Department of Biological Sciences, Smith College, Northampton, MA, USA.; Computational and Systems Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Schneider C; Department of Biological Sciences, Smith College, Northampton, MA, USA.; McGill University, Montreal, QC, Canada., PenkoffLidbeck N; Department of Biological Sciences, Smith College, Northampton, MA, USA., Bashiruddin S; Department of Biological Sciences, Smith College, Northampton, MA, USA.; Family Medicine Assoc, Westfield, MA, USA., Schwartz MS; Department of Biological Sciences, Smith College, Northampton, MA, USA.; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA., Alligood K; Department of Biological Sciences, Smith College, Northampton, MA, USA.; Farmers Conservation Alliance, Hood River, OR, USA., Devoto SH; Biology Department, Wesleyan University, Middletown, CT, USA., Barresi MJF; Department of Molecular and Cellular Biology, University of Massachusetts, Amherst, MA, USA.; Department of Biological Sciences, Smith College, Northampton, MA, USA.
Jazyk:	angličtina
Zdroj:	Developmental neurobiology [Dev Neurobiol] 2021 Jul; Vol. 81 (5), pp. 671-695. Date of Electronic Publication: 2021 Apr 03.
DOI:	10.1002/dneu.22801
Abstrakt:	During embryonic development of bilaterally symmetrical organisms, neurons send axons across the midline at specific points to connect the two halves of the nervous system with a commissure. Little is known about the cells at the midline that facilitate this tightly regulated process. We exploit the conserved process of vertebrate embryonic development in the zebrafish model system to elucidate the identity of cells at the midline that may facilitate postoptic (POC) and anterior commissure (AC) development. We have discovered that three different gfap+ astroglial cell morphologies persist in contact with pathfinding axons throughout commissure formation. Similarly, olig2+ progenitor cells occupy delineated portions of the postoptic and anterior commissures where they act as multipotent, neural progenitors. Moreover, we conclude that both gfap+ and olig2+ progenitor cells give rise to neuronal populations in both the telencephalon and diencephalon; however, these varied cell populations showed significant developmental timing differences between the telencephalon and diencephalon. Lastly, we also showed that fli1a+ mesenchymal cells migrate along the presumptive commissure regions before and during midline axon crossing. Furthermore, following commissure maturation, specific blood vessels formed at the midline of the POC and immediately ventral and parallel to the AC. This comprehensive account of the cellular populations that correlate with the timing and position of commissural axon pathfinding has supported the conceptual modeling and identification of the early forebrain architecture that may be necessary for proper commissure development. (© 2020 Wiley Periodicals, LLC.)
Databáze:	MEDLINE
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