Isolation, characterization and quantitative HPLC-DAD analysis of components of charantin from fruits of Momordica charantia.

Autor: Desai S; Department of Pharmaceutical Quality Assurance, SSR College of Pharmacy, Sayli Road, Silvassa, UT of Dadra and Nagar Haveli 396 230, India., Tatke P; Department of Pharmaceutical Chemistry, C. U. Shah College of Pharmacy, S.N.D.T. Women's University, Santacruz (W), Mumbai 400 049, India. Electronic address: drpratimatatke@gmail.com., Mane T; Scientist 3, Research & Development, Johnson & Johnson Pvt Ltd, Mumbai 400 080, Maharashtra, India., Gabhe S; Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane, Pune 411 038, India.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2021 May 30; Vol. 345, pp. 128717. Date of Electronic Publication: 2020 Nov 25.
DOI: 10.1016/j.foodchem.2020.128717
Abstrakt: Charantin, a steroidal glycoside, exists as a mixture of stigmasterol glucoside (STG) and β-sitosterol glucoside (BSG) in the fruits of Momordica charantia. Charantin has anti-diabetic activity comparable to insulin. The present work discusses a method for separation of components of charantin namely STG and BSG by simple extraction technique followed by preparative HPLC. The identity of separated components was established by chromatographic as well as spectral techniques. Also reversed phase HPLC-DAD method was developed and validated for estimation of STG and BSG present in fruits of Momordica charantia. The method used C18 column (75 mm × 4.6 mm, 3.5 µm) as stationary phase and methanol: water (98:02, v/v) as mobile phase. Retention times of STG and BSG were found to be 10.707 min and 11.870 min, respectively. The validated method was applied to evaluate content of these components in different extracts and some commercial herbal formulations containing fruits of Momordica charantia.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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