Preclinical approaches in vulvovaginal candidiasis treatment with mucoadhesive thermoresponsive systems containing propolis.

Autor: Bonfim AP; Medical Mycology Laboratory, Clinical Analyzes and Biomedicine Department, State University of Maringa, Maringa, PR, Brazil., Sakita KM; Medical Mycology Laboratory, Clinical Analyzes and Biomedicine Department, State University of Maringa, Maringa, PR, Brazil., Faria DR; Medical Mycology Laboratory, Clinical Analyzes and Biomedicine Department, State University of Maringa, Maringa, PR, Brazil., Arita GS; Medical Mycology Laboratory, Clinical Analyzes and Biomedicine Department, State University of Maringa, Maringa, PR, Brazil., Vendramini FAVR; Medical Mycology Laboratory, Clinical Analyzes and Biomedicine Department, State University of Maringa, Maringa, PR, Brazil., Capoci IRG; Medical Mycology Laboratory, Clinical Analyzes and Biomedicine Department, State University of Maringa, Maringa, PR, Brazil., Braga AG; Medical Mycology Laboratory, Clinical Analyzes and Biomedicine Department, State University of Maringa, Maringa, PR, Brazil., Dos Santos RS; Department of Pharmacy, State University of Maringa, Maringa, PR, Brazil., Bruschi ML; Department of Pharmacy, State University of Maringa, Maringa, PR, Brazil., Becker TCA; General Pathology Laboratory, Healthy Basic Sciences Department, State University of Maringa, Maringa, PR, Brazil., de Oliveira Junior AG; Electron Microscopy and Microanalysis Laboratory, State University of Londrina, Londrina, PR, Brazil., Kioshima ÉS; Medical Mycology Laboratory, Clinical Analyzes and Biomedicine Department, State University of Maringa, Maringa, PR, Brazil., de Souza Bonfim-Mendonça P; Medical Mycology Laboratory, Clinical Analyzes and Biomedicine Department, State University of Maringa, Maringa, PR, Brazil., Svidzinski TIE; Medical Mycology Laboratory, Clinical Analyzes and Biomedicine Department, State University of Maringa, Maringa, PR, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2020 Dec 11; Vol. 15 (12), pp. e0243197. Date of Electronic Publication: 2020 Dec 11 (Print Publication: 2020).
DOI: 10.1371/journal.pone.0243197
Abstrakt: Vulvovaginal candidiasis (VVC) is a common vaginitis that affects women, especially in childbearing age, caused by Candida albicans in almost 80% of cases. Considering the limited drug arsenal available and the increasing fungal resistance profile, the search for new therapeutic sources with low toxicity and easy administration should be supported. Propolis has been used as a traditional medicine for multiple diseases, considering its particular composition and pharmaceutical properties that permits its wide applicability; it has also emerged as a potential antifungal agent. Thus, this study performed an in vitro and in vivo investigation into the efficacy of a new mucoadhesive thermoresponsive platform for propolis delivery (MTS-PRPe) in a preclinical murine model of VVC treatment caused by C. albicans. The methodologies involved chemical analysis, an assessment of the rheological and mucoadhesive properties of propolis formulations, in vitro and in vivo antifungal evaluations, histological evaluations and electron microscopy of the vaginal mucosa. The results demonstrated the antifungal activity of propolis extract and MTS-PRP against the standard strain and a fluconazole-resistant clinical isolate of C. albicans, in both in vitro and in vivo assays. These results were similar and even better, depending on the propolis concentration, when compared to nystatin. Thus, the formulation containing propolis exhibited good performance against C. albicans in a vulvovaginal candidiasis experimental model, representing a promising opportunity for the treatment of this infection.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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