A three-decade survey of Brazilian Fusarium oxysporum f. sp. lycopersici races assessed by pathogenicity tests on differential tomato accessions and by molecular markers.

Autor: Gonçalves AM; Departamento de Fitopatologia, Universidade de Brasília (UnB), Brasília-DF, Brazil., Cabral CS; Departamento de Fitopatologia, Universidade de Brasília (UnB), Brasília-DF, Brazil., Reis A; National Center for Vegetable Crops Research (CNPH), Embrapa Hortaliças, Brasília-DF, Brazil., Fonseca MEN; National Center for Vegetable Crops Research (CNPH), Embrapa Hortaliças, Brasília-DF, Brazil., Costa H; Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural (INCAPER), Venda Nova do Imigrante-ES, Brazil., Ribeiro FHS; National Center for Vegetable Crops Research (CNPH), Embrapa Hortaliças, Brasília-DF, Brazil., Boiteux LS; Departamento de Fitopatologia, Universidade de Brasília (UnB), Brasília-DF, Brazil.; National Center for Vegetable Crops Research (CNPH), Embrapa Hortaliças, Brasília-DF, Brazil.
Jazyk: angličtina
Zdroj: Journal of applied microbiology [J Appl Microbiol] 2021 Aug; Vol. 131 (2), pp. 873-884. Date of Electronic Publication: 2021 Jan 05.
DOI: 10.1111/jam.14966
Abstrakt: Aim: Physiological race determination of 143 Fusarium oxysporum f. sp. lycopersici (FOL) isolates collected along 30 years in major tomato-producing regions of Brazil.
Materials and Results: Physiological races were determined via root-dipping inoculation of differential tomato accessions and by the PCR-based marker system of Hirano and Arie (2006). According to pathogenicity/virulence assays, five race 1, 23 race 2 and 115 race 3 isolates were identified. FOL race 1 and 2 isolates prevailed up to early 2000s. Afterwards, the large majority of the isolates was classified as the invasive race 3. Novel reports of race 3 were done in five states, thus expanding its geographical distribution. Using this PCR-based marker system, a precise discrimination was observed for all race 3 isolates. However, all race 1 and 2 isolates displayed only the cosmopolitan race 1-specific amplicon pattern.
Conclusion: The development and/or validation of novel race-specific marker systems are necessary to allow a precise discrimination of the potentially endemic Brazilian FOL race 2.
Significance and Impact of the Study: The present characterization of isolates indicates that distinct evolutionary mechanisms are acting to select new FOL races and/or genetic variants across agroecosystems around the globe.
(© 2020 The Society for Applied Microbiology.)
Databáze: MEDLINE
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