Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients.

Autor: Ortega FG; Balearic Islands Health Research Institute (IdISBa), 07010 Palma de Mallorca, Spain., Regiart MD; LSEME, Laboratório de Sensores Eletroquímicos e Métodos Eletroanalíticos. Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Professor Lineu Prestes, 748, 05513-970 São Paulo, São Paulo, Brazil., Rodríguez-Martínez A; Liquid Biopsy and Metastasis Research Group, GENYO, Centre for Genomics and Oncological Research: Pfizer, University of Granada, Andalusian Regional Government PTS, Granada, Avenida de la Ilustración, 114, 18016 Granada, Spain.; Laboratory of Genetic Identification, Legal Medicine and Toxicology Department, Faculty of Medicine-PTS, University of Granada, Avenida de la Investigación, 11, 18016 Granada, Spain., de Miguel-Pérez D; Liquid Biopsy and Metastasis Research Group, GENYO, Centre for Genomics and Oncological Research: Pfizer, University of Granada, Andalusian Regional Government PTS, Granada, Avenida de la Ilustración, 114, 18016 Granada, Spain., Serrano MJ; Liquid Biopsy and Metastasis Research Group, GENYO, Centre for Genomics and Oncological Research: Pfizer, University of Granada, Andalusian Regional Government PTS, Granada, Avenida de la Ilustración, 114, 18016 Granada, Spain., Lorente JA; Liquid Biopsy and Metastasis Research Group, GENYO, Centre for Genomics and Oncological Research: Pfizer, University of Granada, Andalusian Regional Government PTS, Granada, Avenida de la Ilustración, 114, 18016 Granada, Spain.; Laboratory of Genetic Identification, Legal Medicine and Toxicology Department, Faculty of Medicine-PTS, University of Granada, Avenida de la Investigación, 11, 18016 Granada, Spain., Tortella G; Departamento de Ingeniería Química, Universidad de La Frontera, 4811230 Temuco, Chile.; Centro de Excelencia en Investigación Biotecnológica Aplicada al Medio Ambiente (CIBAMA), 4811230 Temuco, Chile., Rubilar O; Departamento de Ingeniería Química, Universidad de La Frontera, 4811230 Temuco, Chile.; Centro de Excelencia en Investigación Biotecnológica Aplicada al Medio Ambiente (CIBAMA), 4811230 Temuco, Chile., Sapag K; INFAP, Laboratorio de Solidos Porosos, Universidad Nacional de San Luis, CONICET, Ejercito de los Andes 950, D5700BWS San Luis, Argentina., Bertotti M; LSEME, Laboratório de Sensores Eletroquímicos e Métodos Eletroanalíticos. Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Professor Lineu Prestes, 748, 05513-970 São Paulo, São Paulo, Brazil., Fernández-Baldo MA; INQUISAL, Departamento de Química, Universidad Nacional de San Luis, CONICET, Chacabuco 917, D5700BWS San Luis, Argentina.
Jazyk: angličtina
Zdroj: Analytical chemistry [Anal Chem] 2021 Jan 19; Vol. 93 (2), pp. 1143-1153. Date of Electronic Publication: 2020 Dec 10.
DOI: 10.1021/acs.analchem.0c04180
Abstrakt: This study is focused on identifying novel epithelial markers in circulating extracellular vesicles (EVs) through the development of a dual sandwich-type electrochemical paper-based immunosensor for Claudin 7 and CD81 determination, as well as its validation in breast cancer (BC) patients. This immunosensor allows for rapid, sensitive, and label-free detection of these two relevant BC biomarkers. Under optimum conditions, the limit of detection for Claudin 7 was 0.4 pg mL -1 , with a wide linear range of 2 to 1000 pg mL -1 , while for CD81, the limit of detection was 3 pg mL -1 , with a wide linear range of 0.01 to 10 ng mL -1 . Finally, we validated Claudin 7 and CD81 determination in EVs from 60 BC patients and 20 healthy volunteers, reporting higher diagnostic accuracy than the one observed with classical diagnostic markers. This analysis provides a low-cost, specific, versatile, and user-friendly strategy as a robust and reliable tool for early BC diagnosis.
Databáze: MEDLINE
Externí odkaz: