Extracellular-Matrix-Reinforced Bioinks for 3D Bioprinting Human Tissue.

Autor: De Santis MM; Lung Bioengineering and Regeneration, Dept of Experimental Medical Sciences, Stem Cell Centre, Wallenberg Center for Molecular Medicine, Lund University, Lund, 22362, Sweden.; Research Unit Lung Repair and Regeneration, Helmholtz Zentrum München, German Research Center for Environmental Health, Ludwig-Maximilians-University, University Hospital Grosshadern, Member of the German Center of Lung Research (DZL), Munich, 81377, Germany., Alsafadi HN; Lung Bioengineering and Regeneration, Dept of Experimental Medical Sciences, Stem Cell Centre, Wallenberg Center for Molecular Medicine, Lund University, Lund, 22362, Sweden., Tas S; Lung Bioengineering and Regeneration, Dept of Experimental Medical Sciences, Stem Cell Centre, Wallenberg Center for Molecular Medicine, Lund University, Lund, 22362, Sweden., Bölükbas DA; Lung Bioengineering and Regeneration, Dept of Experimental Medical Sciences, Stem Cell Centre, Wallenberg Center for Molecular Medicine, Lund University, Lund, 22362, Sweden., Prithiviraj S; Laboratory for Cell, Tissue and Organ Engineering, Dept of Clinical Sciences Lund, Stem Cell Centre, Wallenberg Center for Molecular Medicine, Lund University, Lund, 22362, Sweden., Da Silva IAN; Lung Bioengineering and Regeneration, Dept of Experimental Medical Sciences, Stem Cell Centre, Wallenberg Center for Molecular Medicine, Lund University, Lund, 22362, Sweden., Mittendorfer M; Lung Bioengineering and Regeneration, Dept of Experimental Medical Sciences, Stem Cell Centre, Wallenberg Center for Molecular Medicine, Lund University, Lund, 22362, Sweden., Ota C; Research Unit Lung Repair and Regeneration, Helmholtz Zentrum München, German Research Center for Environmental Health, Ludwig-Maximilians-University, University Hospital Grosshadern, Member of the German Center of Lung Research (DZL), Munich, 81377, Germany., Stegmayr J; Lung Bioengineering and Regeneration, Dept of Experimental Medical Sciences, Stem Cell Centre, Wallenberg Center for Molecular Medicine, Lund University, Lund, 22362, Sweden., Daoud F; Department of Experimental Medical Science, Lund University, Lund, 22362, Sweden., Königshoff M; Research Unit Lung Repair and Regeneration, Helmholtz Zentrum München, German Research Center for Environmental Health, Ludwig-Maximilians-University, University Hospital Grosshadern, Member of the German Center of Lung Research (DZL), Munich, 81377, Germany., Swärd K; Department of Experimental Medical Science, Lund University, Lund, 22362, Sweden., Wood JA; Soft Matter, Fluidics and Interfaces, MESA+ Institute for Nanotechnology, University of Twente, Enschede, 7522, The Netherlands., Tassieri M; Division of Biomedical Engineering, James Watt School of Engineering, University of Glasgow, Glasgow, G12 8LT, United Kingdom., Bourgine PE; Laboratory for Cell, Tissue and Organ Engineering, Dept of Clinical Sciences Lund, Stem Cell Centre, Wallenberg Center for Molecular Medicine, Lund University, Lund, 22362, Sweden., Lindstedt S; Dept of Cardiothoracic Surgery, Heart and Lung Transplantation, Wallenberg Center for Molecular Medicine, Lund University Hospital, Lund, 22242, Sweden., Mohlin S; Division of Pediatrics, Clinical Sciences, Translational Cancer Research, Lund University Cancer Center at Medicon Village, Lund, 22363, Sweden., Wagner DE; Lung Bioengineering and Regeneration, Dept of Experimental Medical Sciences, Stem Cell Centre, Wallenberg Center for Molecular Medicine, Lund University, Lund, 22362, Sweden.; Research Unit Lung Repair and Regeneration, Helmholtz Zentrum München, German Research Center for Environmental Health, Ludwig-Maximilians-University, University Hospital Grosshadern, Member of the German Center of Lung Research (DZL), Munich, 81377, Germany.
Jazyk: angličtina
Zdroj: Advanced materials (Deerfield Beach, Fla.) [Adv Mater] 2021 Jan; Vol. 33 (3), pp. e2005476. Date of Electronic Publication: 2020 Dec 09.
DOI: 10.1002/adma.202005476
Abstrakt: Recent advances in 3D bioprinting allow for generating intricate structures with dimensions relevant for human tissue, but suitable bioinks for producing translationally relevant tissue with complex geometries remain unidentified. Here, a tissue-specific hybrid bioink is described, composed of a natural polymer, alginate, reinforced with extracellular matrix derived from decellularized tissue (rECM). rECM has rheological and gelation properties beneficial for 3D bioprinting while retaining biologically inductive properties supporting tissue maturation ex vivo and in vivo. These bioinks are shear thinning, resist cell sedimentation, improve viability of multiple cell types, and enhance mechanical stability in hydrogels derived from them. 3D printed constructs generated from rECM bioinks suppress the foreign body response, are pro-angiogenic and support recipient-derived de novo blood vessel formation across the entire graft thickness in a murine model of transplant immunosuppression. Their proof-of-principle for generating human tissue is demonstrated by 3D bioprinting human airways composed of regionally specified primary human airway epithelial progenitor and smooth muscle cells. Airway lumens remained patent with viable cells for one month in vitro with evidence of differentiation into mature epithelial cell types found in native human airways. rECM bioinks are a promising new approach for generating functional human tissue using 3D bioprinting.
(© 2020 The Authors. Advanced Materials published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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