The immune gene expression induced by Cooperia punctata in naturally infected calves with resistance and susceptible phenotype traits.

Autor: Matamoros-Mercado I; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad. Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Carr. Fed. Cuernavaca-Cuautla#8534, Jiutepec, Mor., C.P. 62550, Mexico., von-Son-de-Fernex E; Centro de Enseñanza, Investigación y Extensión en Ganadería Tropical, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Km 5.5. Carr. Fed., Tlapacoyan-Martínez de la Torre, Veracruz, C.P. 93600, Mexico., Alonso-Díaz MÁ; Centro de Enseñanza, Investigación y Extensión en Ganadería Tropical, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Km 5.5. Carr. Fed., Tlapacoyan-Martínez de la Torre, Veracruz, C.P. 93600, Mexico., Reyes-Guerrero DE; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad. Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Carr. Fed. Cuernavaca-Cuautla#8534, Jiutepec, Mor., C.P. 62550, Mexico., Olazaran-Jenkins S; Sitio Experimental Las Margaritas, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Hueytamalco, Pue., Mexico., Sánchez-Sánchez B; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad. Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Carr. Fed. Cuernavaca-Cuautla#8534, Jiutepec, Mor., C.P. 62550, Mexico., López-Arellano ME; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad. Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Carr. Fed. Cuernavaca-Cuautla#8534, Jiutepec, Mor., C.P. 62550, Mexico. Electronic address: lopez.mariaeugenia@inifap.gob.mx.
Jazyk: angličtina
Zdroj: Veterinary parasitology [Vet Parasitol] 2021 Jan; Vol. 289, pp. 109325. Date of Electronic Publication: 2020 Nov 21.
DOI: 10.1016/j.vetpar.2020.109325
Abstrakt: The analysis of the immune gene expression was performed in Zebu × Holstein calves with resistant and susceptible phenotypes naturally infected with Cooperia punctata. Fourteen calves of 4 months old were grazed for 11 weeks under a tropical climate. The parasitic infection showed an average epg value of 1055 ± 1155 and an IgG optical density of 0.814 ± 0.0.037 with statistic differences among the different weeks (p < 0.05), and a pcv value of 24 ± 2.0 % (p > 0.05). High variation in epg value was observed, between 7 ± 7.14 and 4657 ± 1886, and, based on these differences; the infected hosts were classified as five resistant calves with epg ≤ 200 and nine susceptible calves with epg ≥ 300. Moreover, IgG levels displayed statistical differences between resistance and susceptible calves to C. punctata infection. The immune gene expression was analysed in three resistant and susceptible calves, respectively. Nine cytokine genes and the FCεR1A receptor were analysed at the 3rd and 11th weeks post-infection. In the first period upregulation was found, from 2.19- to 9.45-fold, (p < 0.05) for IL-2, -5, - 6, -10, TGF-β and FCεR1A in the resistant group; the expression was decreased at the 11th week with low level of IgG. In contrast, downregulation for susceptible calves was found for nine immune genes and upregulation for INF-γ in both periods together with increased IgG levels. In conclusion, immune gene expression was regulated at the begging infection of C. punctata in resistant grazing calves. In contrast, suppression of important genes was involved in calves susceptible to C. punctata.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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