Resistotyping and extended-spectrum beta-lactamase genes among Escherichia coli from wastewater treatment plants and recipient surface water for reuse in South Africa.

Autor: Nzima B; Institute for Water and Wastewater Technology, Durban University of Technology, Durban, South Africa., Adegoke AA; Institute for Water and Wastewater Technology, Durban University of Technology, Durban, South Africa.; Department of Microbiology, Faculty of Science, University of Uyo, Uyo, Akwa Ibom State, Nigeria., Ofon UA; Department of Microbiology, Faculty of Science, University of Uyo, Uyo, Akwa Ibom State, Nigeria., Al-Dahmoshi HOM; Biology Department, College of Science, University of Babylon, Babylon Province-Hilla City, Iraq., Saki M; Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.; Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran., Ndubuisi-Nnaji UU; Department of Microbiology, Faculty of Science, University of Uyo, Uyo, Akwa Ibom State, Nigeria., Inyang CU; Department of Microbiology, Faculty of Science, University of Uyo, Uyo, Akwa Ibom State, Nigeria.
Jazyk: angličtina
Zdroj: New microbes and new infections [New Microbes New Infect] 2020 Oct 31; Vol. 38, pp. 100803. Date of Electronic Publication: 2020 Oct 31 (Print Publication: 2020).
DOI: 10.1016/j.nmni.2020.100803
Abstrakt: The spread of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli has increased in different environments. This study aimed to evaluate the patterns of antibiotic resistance and ESBL genes among E. coli isolates collected from wastewater and recipient surface water in South Africa. Fifteen samples containing nine wastewater and six river water samples were collected from a local wastewater treatment plant. The E. coli isolates were detected using standard microbiology methods. Antibiotic susceptibility testing was performed using disc diffusion agar. The occurrence of bla CTX-M , bla SHV and bla TEM ESBL genes was investigated by PCR. Exactly 140 isolates were selected from the primary enumeration plates with a log 10  CFU/mL count that ranged from 4.1 to 4.2 (influent), 4.2 to 4.5 (biofilter) and 2.5 to 3.3 (effluent). The wastewater effluent showed an impact on the receiving water environment, as the treatment efficiency was 92% and the downstream log 10  CFU/mL count (range, 3.6-3.8 log 10  CFU/mL) was higher than the upstream count (range, 3.3-3.6 log 10  CFU/mL). Antibiotic testing results showed that 40% to 100% of E. coli isolates were resistant to ampicillin, penicillin, tetracycline and cefotaxime but susceptible to imipenem, meropenem and ciprofloxacin. A total of 40 studied isolates (28.6%) had both the bla TEM and bla CTX-M genes, while no bla SHV was detected. The wastewater treatment plants contributed multidrug-resistant ESBL-producing E. coli isolates that can be potential environmental health risks. Regular monitoring policies are recommended to prevent the spread of antibiotic resistance in the region.
(© 2020 The Authors.)
Databáze: MEDLINE
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