Dietary exposure to mercury and its relation to cytogenetic instability in populations from "La Mojana" region, northern Colombia.

Autor: Galeano-Páez C; Facultad de Ciencias de La Salud, Laboratorio de Investigación Biomédica y Biología Molecular, Universidad Del Sinú, Montería, Córdoba, Colombia., Espitia-Pérez P; Facultad de Ciencias de La Salud, Laboratorio de Investigación Biomédica y Biología Molecular, Universidad Del Sinú, Montería, Córdoba, Colombia. Electronic address: pedrojespitia@unisinu.edu.co., Jimenez-Vidal L; Facultad de Ciencias de La Salud, Laboratorio de Investigación Biomédica y Biología Molecular, Universidad Del Sinú, Montería, Córdoba, Colombia., Pastor-Sierra K; Facultad de Ciencias de La Salud, Laboratorio de Investigación Biomédica y Biología Molecular, Universidad Del Sinú, Montería, Córdoba, Colombia., Salcedo-Arteaga S; Facultad de Ciencias de La Salud, Laboratorio de Investigación Biomédica y Biología Molecular, Universidad Del Sinú, Montería, Córdoba, Colombia., Hoyos-Giraldo LS; Departamento de Biología, Grupo de Investigación en Genética Toxicológica y Citogenética, Facultad de Ciencias Naturales y Exactas de La Educación, Universidad Del Cauca, Popayán, Cauca, Colombia., Gioda A; Departamento de Química, Pontifícia Universidade Católica Do Rio de Janeiro (PUC-Rio), Rio de Janeiro, Rio de Janeiro, Brazil., Saint'Pierre TD; Departamento de Química, Pontifícia Universidade Católica Do Rio de Janeiro (PUC-Rio), Rio de Janeiro, Rio de Janeiro, Brazil., García SC; Laboratório de Toxicologia (LATOX), Universidade Federal Do Rio Grande Do Sul, Porto Alegre, Rio Grande do Sul, Brazil., Brango H; Departamento de Matemáticas y Estadística, Facultad de Ciencias Básicas, Universidad de Córdoba, Montería, Córdoba, Colombia., Espitia-Pérez L; Facultad de Ciencias de La Salud, Laboratorio de Investigación Biomédica y Biología Molecular, Universidad Del Sinú, Montería, Córdoba, Colombia. Electronic address: lydaespitia@unisinu.edu.co.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2021 Feb; Vol. 265, pp. 129066. Date of Electronic Publication: 2020 Nov 21.
DOI: 10.1016/j.chemosphere.2020.129066
Abstrakt: Fish consumption and chronic exposure to low doses of mercury (Hg) seems to activate several molecular mechanisms leading to carcinogenic and/or teratogenic processes. However, Hg genotoxic effects on humans are not completely described. In the present study, we assessed cytogenetic damage in isolated human peripheral lymphocytes using the cytokinesis-block micronucleus cytome assay (CBMN-Cyt), micronucleus formation with anti-kinetochore antibody (CREST staining), levels of total Hg in hair (T-Hg), fish consumption, and estimated Hg dose. The study comprised 39 non-exposed, and 73 residents from La Mojana region, an area with a well-documented Hg contamination. Data showed a significant increase in micronuclei (MNBN), nucleoplasmic bridges (NPB), and necrotic and apoptotic cell frequencies in residents of "La Mojana." The overall mean T-Hg level in hair for exposed residents was 1.12 ± 0.94 mg kg -1 and 0.15 ± 0.05 in individuals from the reference area. Approximately 40% of analyzed individuals showed T-Hg levels that exceeded US Environmental Protection Agency (USEPA) reference dose. Increased T-Hg levels in hair were related to increased MNBN frequencies and high fish consumption. Other cellular markers, such as necrotic and apoptotic cell frequencies, were also correlated with high fish intake and T-Hg contents. Results of the CREST staining demonstrated that in vivo exposure to Hg induces genetic instability by chromosome fragment loss (clastogenic). Additionally, a high average intake of some fish species, particularly with carnivorous habits like Caquetaia kraussii, Hoplias malabaricus, and Sorubin cuspicaudus, seems to increase MNBN frequencies significantly.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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