Proteomic Study Identifies Glycolytic and Inflammation Pathways Involved in Recurrent Otitis Media.

Autor: Ura B; Institute for Maternal and Child Health-IRCCS 'Burlo Garofolo', 65/1 Via dell'Istria, 34137 Trieste, Italy., Celsi F; Institute for Maternal and Child Health-IRCCS 'Burlo Garofolo', 65/1 Via dell'Istria, 34137 Trieste, Italy., Zupin L; Institute for Maternal and Child Health-IRCCS 'Burlo Garofolo', 65/1 Via dell'Istria, 34137 Trieste, Italy., Arrigoni G; Department of Biomedical Sciences, University of Padova, Via U. Bassi 58/B, 35121 Padova, Italy.; Proteomics Center, University of Padova and Azienda Ospedaliera di Padova, Via G. Orus 2/B, 35129 Padova, Italy.; CRIBI Biotechnology Center, University of Padova, Via U. Bassi 58/B, 35121 Padova, Italy., Battisti I; Department of Biomedical Sciences, University of Padova, Via U. Bassi 58/B, 35121 Padova, Italy.; Proteomics Center, University of Padova and Azienda Ospedaliera di Padova, Via G. Orus 2/B, 35129 Padova, Italy., Gaita B; Institute for Maternal and Child Health-IRCCS 'Burlo Garofolo', 65/1 Via dell'Istria, 34137 Trieste, Italy., Grasso DL; Institute for Maternal and Child Health-IRCCS 'Burlo Garofolo', 65/1 Via dell'Istria, 34137 Trieste, Italy., Orzan E; Institute for Maternal and Child Health-IRCCS 'Burlo Garofolo', 65/1 Via dell'Istria, 34137 Trieste, Italy., Sagredini R; Institute for Maternal and Child Health-IRCCS 'Burlo Garofolo', 65/1 Via dell'Istria, 34137 Trieste, Italy., Barbi E; Institute for Maternal and Child Health-IRCCS 'Burlo Garofolo', 65/1 Via dell'Istria, 34137 Trieste, Italy.; Department of Medical, Surgery and Health Sciences, University of Trieste, 34149 Trieste, Italy., Crovella S; Department of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University-Women's College of Sciences Building, Doha 2713, Qatar.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2020 Dec 05; Vol. 21 (23). Date of Electronic Publication: 2020 Dec 05.
DOI: 10.3390/ijms21239291
Abstrakt: Recurrent acute otitis media (RAOM) in children is clinically defined as the occurrence of at least three episodes of acute otitis media over a course of 6 months. A further common pathological condition of interest in the context of pediatric otolaryngology is adenotonsillar hypertrophy (ATH), a common cause of obstructive sleep apnea syndrome. Aimed at unraveling the differential modulation of proteins in the two pathologies and at understanding the possible pathways involved in their onset, we analyzed the proteomic profile of the adenoids from 14 RAOM and ATH patients by using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). The 2-DE coupled with MS allowed us to identify 23 spots with significant ( p -value < 0.05) changes in protein amount, recognizing proteins involved in neutrophil degranulation and glycolysis pathways.
Databáze: MEDLINE
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