Expression genome-wide association study identifies that phosphatidylinositol-derived signalling regulates ALUMINIUM SENSITIVE3 expression under aluminium stress in the shoots of Arabidopsis thaliana.

Autor: Sadhukhan A; Applied Biological Sciences, Gifu University, Yanagido 1-1, Gifu, Gifu 501-1193, Japan., Agrahari RK; Applied Biological Sciences, Gifu University, Yanagido 1-1, Gifu, Gifu 501-1193, Japan., Wu L; Applied Biological Sciences, Gifu University, Yanagido 1-1, Gifu, Gifu 501-1193, Japan., Watanabe T; Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kitaku, Sapporo, 060-8589, Japan., Nakano Y; Applied Biological Sciences, Gifu University, Yanagido 1-1, Gifu, Gifu 501-1193, Japan., Panda SK; Department of Biochemistry, Central University of Rajasthan, Rajasthan 305817, India., Koyama H; Applied Biological Sciences, Gifu University, Yanagido 1-1, Gifu, Gifu 501-1193, Japan., Kobayashi Y; Applied Biological Sciences, Gifu University, Yanagido 1-1, Gifu, Gifu 501-1193, Japan. Electronic address: k_yuriko@gifu-u.ac.jp.
Jazyk: angličtina
Zdroj: Plant science : an international journal of experimental plant biology [Plant Sci] 2021 Jan; Vol. 302, pp. 110711. Date of Electronic Publication: 2020 Oct 14.
DOI: 10.1016/j.plantsci.2020.110711
Abstrakt: To identify unknown regulatory mechanisms leading to aluminium (Al)-induction of the Al tolerance gene ALS3, we conducted an expression genome-wide association study (eGWAS) for ALS3 in the shoots of 95 Arabidopsis thaliana accessions in the presence of Al. The eGWAS was conducted using a mixed linear model with 145,940 genome-wide single nucleotide polymorphisms (SNPs) and the association results were validated using reverse genetics. We found that many SNPs from the eGWAS were associated with genes related to phosphatidylinositol metabolism as well as stress signal transduction, including Ca 2+ signals, inter-connected in a co-expression network. Of these, PLC9, CDPK32, ANAC071, DIR1, and a hypothetical protein (AT4G10470) possessed amino acid sequence/ gene expression level polymorphisms that were significantly associated with ALS3 expression level variation. Furthermore, T-DNA insertion mutants of PLC9, CDPK32, and ANAC071 suppressed shoot ALS3 expression in the presence of Al. This study clarified the regulatory mechanisms of ALS3 expression in the shoot and provided genetic evidence of the involvement of phosphatidylinositol-derived signal transduction under Al stress.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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