| Autor: |
Kononikhin AS; Skolkovo Institute of Science and Technology, 143026 Moscow, Russia.; V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, 117198 Moscow, Russia., Zakharova NV; Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia., Sergeeva VA; Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia.; V. L. Talrose Institute for Energy Problems of Chemical Physics, N. N. Semenov Federal Center of Chemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia., Indeykina MI; Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia., Starodubtseva NL; V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, 117198 Moscow, Russia.; Moscow Institute of Physics and Technology, 141701 Moscow, Russia., Bugrova AE; V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, 117198 Moscow, Russia.; Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia., Muminova KT; V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, 117198 Moscow, Russia., Khodzhaeva ZS; V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, 117198 Moscow, Russia., Popov IA; V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, 117198 Moscow, Russia.; Moscow Institute of Physics and Technology, 141701 Moscow, Russia., Shao W; Institute of Molecular Systems Biology, ETH Zurich, 8092 Zurich, Switzerland., Pedrioli P; Institute of Molecular Systems Biology, ETH Zurich, 8092 Zurich, Switzerland., Shmakov RG; V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, 117198 Moscow, Russia., Frankevich VE; V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, 117198 Moscow, Russia., Sukhikh GT; V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, 117198 Moscow, Russia., Nikolaev EN; Skolkovo Institute of Science and Technology, 143026 Moscow, Russia. |
| Abstrakt: |
Preeclampsia (PE) is a severe pregnancy complication, which may be considered as a systemic response in the second half of pregnancy to physiological failures in the first trimester, and can lead to very serious consequences for the health of the mother and fetus. Since PE is often associated with proteinuria, urine proteomic assays may represent a powerful tool for timely diagnostics and appropriate management. High resolution mass spectrometry was applied for peptidome analysis of 127 urine samples of pregnant women with various hypertensive complications: normotensive controls ( n = 17), chronic hypertension ( n = 16), gestational hypertension ( n = 15), mild PE ( n = 25), severe PE ( n = 25), and 29 patients with complicated diagnoses. Analysis revealed 3869 peptides, which mostly belong to 116 groups with overlapping sequences. A panel of 22 marker peptide groups reliably differentiating PE was created by multivariate statistics, and included 15 collagen groups (from COL1A1, COL3A1, COL2A1, COL4A4, COL5A1, and COL8A1), and single loci from alpha-1-antitrypsin, fibrinogen, membrane-associated progesterone receptor component 1, insulin, EMI domain-containing protein 1, lysine-specific demethylase 6B, and alpha-2-HS-glycoprotein each. ROC analysis of the created model resulted in 88% sensitivity, 96.8% specificity, and receiver operating characteristic curve (AUC) = 0.947. Obtained results confirm the high diagnostic potential of urinary peptidome profiling for pregnancy hypertensive disorders diagnostics. |
