Simple Platform for Automating Decoupled LC-MS Analysis of Hydrogen/Deuterium Exchange Samples.

Autor:	Watson MJ; Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195, United States., Harkewicz R; Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195, United States., Hodge EA; Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195, United States., Vorauer C; Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195, United States., Palmer J; Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195, United States., Lee KK; Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195, United States., Guttman M; Department of Medicinal Chemistry, University of Washington, Seattle, Washington 98195, United States.
Jazyk:	angličtina
Zdroj:	Journal of the American Society for Mass Spectrometry [J Am Soc Mass Spectrom] 2021 Feb 03; Vol. 32 (2), pp. 597-600. Date of Electronic Publication: 2020 Dec 07.
DOI:	10.1021/jasms.0c00341
Abstrakt:	Hydrogen/deuterium exchange with mass spectrometry (HDX-MS) is capable of providing unique insight into complex biological systems that are difficult to study by other techniques. Due to arduous sample handling requirements, automating HDX experimentation for higher throughput requires specialized equipment. While recent advances have enabled automation of sample preparation and analysis, several proteins of interest and types of HDX experiments remain incompatible with automated workflows and require manual sample preparation that greatly limits experimental throughput. To expand throughput and increase the precision of HDX-MS for systems requiring manual preparation, we have developed an inexpensive autosampler capable of thawing and injecting frozen HDX-MS samples in a highly reproducible manner.
Databáze:	MEDLINE
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