Assessment of progressive alterations in collagen organization in the postoperative conjunctiva by multiphoton microscopy.
| Autor: | Seet LF; Ocular Therapeutics and Drug Delivery, Singapore Eye Research Institute, Singapore.; Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.; Duke-NUS Medical School, Singapore.; Co-corresponding authors.; seet.li.fong@seri.com.sg., Chu SWL; Ocular Therapeutics and Drug Delivery, Singapore Eye Research Institute, Singapore.; stephanie.chu.w.l@seri.com.sg., Teng X; Histoindex Pte Ltd, Singapore.; teng.xiao@choututech.com., Toh LZ; Ocular Therapeutics and Drug Delivery, Singapore Eye Research Institute, Singapore.; toh.li.zhen@seri.com.sg., Wong TT; Ocular Therapeutics and Drug Delivery, Singapore Eye Research Institute, Singapore.; Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.; Duke-NUS Medical School, Singapore.; Glaucoma Service, Singapore National Eye Centre, Singapore.; Co-corresponding authors.; tina.wong.t.l@singhealth.com.sg. |
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| Jazyk: | angličtina |
| Zdroj: | Biomedical optics express [Biomed Opt Express] 2020 Oct 19; Vol. 11 (11), pp. 6495-6515. Date of Electronic Publication: 2020 Oct 19 (Print Publication: 2020). |
| DOI: | 10.1364/BOE.403555 |
| Abstrakt: | Glaucoma filtration surgery (GFS) commonly fails due to excessive fibrosis. As collagen structure aberrations is implicated in adverse fibrotic progression, this study aims to uncover collagen organization alterations during postoperative scarring. Via quantitative second harmonic generation/two photon excitation multiphoton imaging, we reveal the scar development and phenotype in the mouse model of conjunctival scarring. We also show that multiphoton imaging corroborated the collagen ultrastructure anomaly characteristic of the SPARC-/- mouse postoperative conjunctiva. These data improve our understanding of postoperative conjunctival scarring and further enhance the utility of this model for the development of anti-fibrotic therapeutics for GFS. Competing Interests: The authors declare no conflicts of interest. (© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.) |
| Databáze: | MEDLINE |
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