Wenzhouxiangella Strain AB-CW3, a Proteolytic Bacterium From Hypersaline Soda Lakes That Preys on Cells of Gram-Positive Bacteria.
| Autor: | Sorokin DY; Winogradsky Institute of Microbiology, Federal Research Centre for Biotechnology, Russian Academy of Sciences, Moscow, Russia.; Department of Biotechnology, Delft University of Technology, Delft, Netherlands., Mosier D; Department of Geoscience, University of Calgary, Calgary, AB, Canada., Zorz JK; Department of Geoscience, University of Calgary, Calgary, AB, Canada., Dong X; Department of Geoscience, University of Calgary, Calgary, AB, Canada., Strous M; Department of Geoscience, University of Calgary, Calgary, AB, Canada. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in microbiology [Front Microbiol] 2020 Nov 13; Vol. 11, pp. 597686. Date of Electronic Publication: 2020 Nov 13 (Print Publication: 2020). |
| DOI: | 10.3389/fmicb.2020.597686 |
| Abstrakt: | A new haloalkaliphilic species of Wenzhouxiangella , strain AB-CW3, was isolated from a system of hypersaline alkaline soda lakes in the Kulunda Steppe using cells of Staphylococcus aureus as growth substrate. AB-CW3's complete, circular genome was assembled from combined nanopore and Illumina sequencing and its proteome was determined for three different experimental conditions. AB-CW3 is an aerobic gammaproteobacterium feeding mainly on proteins and peptides. Unique among Wenzhouxiangella , it uses a flagellum for motility, fimbria for cell attachment and is capable of complete denitrification. AB-CW3 can use proteins derived from living or dead cells of Staphylococcus and other Gram-positive bacteria as the carbon and energy source. It encodes and expresses production of a novel Lantibiotic, a class of antimicrobial peptides which have so far only been found to be produced by Gram-positive bacteria. AB-CW3 likely excretes this peptide via a type I secretion system encoded upstream of the genes for production of the Lanthipeptide. Comparison of AB-CW3's genome to 18 other Wenzhouxiangella genomes from marine, hypersaline, and soda lake habitats indicated one or two transitions from marine to soda lake environments followed by a transition of W. marina back to the oceans. Only 19 genes appear to set haloalkaliphilic Wenzhouxiangella apart from their neutrophilic relatives. As strain AB-CW3 is only distantly related to other members of the genus, we propose to provisionally name it " Wenzhouxiangella alkaliphila ". (Copyright © 2020 Sorokin, Mosier, Zorz, Dong and Strous.) |
| Databáze: | MEDLINE |
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