DNA Terminal-Specific Dispersion Behavior of Polystyrene Latex Microparticles Densely Covered with Oligo-DNA Strands Under High-Salt Conditions.

Autor: Nakauchi H; Department of Biomedical Engineering, Graduate School of Medicine, Science and Technology, Shinshu University, 4-17-1 Wakasato, Nagano, Nagano, 380-8553, Japan., Maeda M; Department of Biomedical Engineering, Graduate School of Medicine, Science and Technology, Shinshu University, 4-17-1 Wakasato, Nagano, Nagano, 380-8553, Japan.; Bioengineering Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan., Kanayama N; Department of Biomedical Engineering, Graduate School of Medicine, Science and Technology, Shinshu University, 4-17-1 Wakasato, Nagano, Nagano, 380-8553, Japan. nkanayma@shinshu-u.ac.jp.; Bioengineering Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan. nkanayma@shinshu-u.ac.jp.; Institute of Biomedical Science, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano, 390-8621, Japan. nkanayma@shinshu-u.ac.jp.
Jazyk: angličtina
Zdroj: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [Anal Sci] 2021 Mar 10; Vol. 37 (3), pp. 461-465. Date of Electronic Publication: 2020 Dec 04.
DOI: 10.2116/analsci.20SCP04
Abstrakt: We prepared microspheres densely covered with oligo-DNA strands by immobilizing amino-terminated oligo-DNA strands on the surface of carboxylate polystyrene latex (PS) particles via the amide bond formation. The obtained microspheres (ssDNA-PS) stably dispersed in neutral pH buffer containing high concentrations of NaCl. For the ssDNA-PS ≥1 μm diameter, only 3 - 5% of surface-immobilized oligo-DNA could form a duplex with the complementary strands. Nevertheless, the resulting ssDNA-PS showed a distinct duplex terminal dependency in their dispersion behavior under neutral pH and high NaCl conditions; the microspheres with fully-matched duplexes on the surface spontaneously aggregated in a non-crosslinking manner. By contrast, the microspheres with terminal-mismatched duplexes remained dispersed under the identical conditions. These results suggest that the micrometer-scale particles covered with oligo-DNA strands also have high susceptibility to a duplex terminal sequence in their dispersion property, similar to previously reported DNA-functionalized nanoparticles. This property could potentially be used in various applications including analytical purposes.
Databáze: MEDLINE
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