A comparison of non-magnetic and magnetic beads for measuring IgG antibodies against Plasmodium vivax antigens in a multiplexed bead-based assay using Luminex technology (Bio-Plex 200 or MAGPIX).

Autor: Mazhari R; Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.; Department of Medical Biology, University of Melbourne, Parkville, Victoria, Australia., Brewster J; Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia., Fong R; Case Western Reserve University, Cleveland, Ohio, United States of America., Bourke C; Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.; Department of Medical Biology, University of Melbourne, Parkville, Victoria, Australia., Liu ZSJ; Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.; Department of Medical Biology, University of Melbourne, Parkville, Victoria, Australia., Takashima E; Division of Malaria Research, Proteo-Science Center, Ehime University, Matsuyama, Japan., Tsuboi T; Division of Malaria Research, Proteo-Science Center, Ehime University, Matsuyama, Japan., Tham WH; Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.; Department of Medical Biology, University of Melbourne, Parkville, Victoria, Australia., Harbers M; CellFree Sciences Co., Ltd., Yokohama, Japan.; RIKEN Center for Integrative Medical Sciences, Yokohama, Japan., Chitnis C; Department of Parasites & Insect Vectors, Malaria Parasite Biology and Vaccines, Institut Pasteur, Paris, France., Healer J; Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.; Department of Medical Biology, University of Melbourne, Parkville, Victoria, Australia., Ome-Kaius M; Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.; Vector Borne Diseases Unit, PNG Institute of Medical Research, Madang, Papua New Guinea., Sattabongkot J; Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand., Kazura J; Case Western Reserve University, Cleveland, Ohio, United States of America., Robinson LJ; Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.; Department of Medical Biology, University of Melbourne, Parkville, Victoria, Australia.; Vector Borne Diseases Unit, PNG Institute of Medical Research, Madang, Papua New Guinea.; Burnet Institute, Melbourne, Australia., King C; Case Western Reserve University, Cleveland, Ohio, United States of America., Mueller I; Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.; Department of Medical Biology, University of Melbourne, Parkville, Victoria, Australia.; Department of Parasites & Insect Vectors, Malaria Parasites & Hosts Unit, Institut Pasteur, Paris, France., Longley RJ; Population Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.; Department of Medical Biology, University of Melbourne, Parkville, Victoria, Australia.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2020 Dec 04; Vol. 15 (12), pp. e0238010. Date of Electronic Publication: 2020 Dec 04 (Print Publication: 2020).
DOI: 10.1371/journal.pone.0238010
Abstrakt: Multiplexed bead-based assays that use Luminex® xMAP® technology have become popular for measuring antibodies against proteins of interest in many fields, including malaria and more recently SARS-CoV-2/COVID-19. There are currently two formats that are widely used: non-magnetic beads or magnetic beads. Data are lacking regarding the comparability of results obtained using these two types of beads, and for assays run on different instruments. Whilst non-magnetic beads can only be run on flow-based instruments (such as the Luminex® 100/200™ or Bio-Plex® 200), magnetic beads can be run on both these and the newer MAGPIX® instruments. In this study we utilized a panel of purified recombinant Plasmodium vivax proteins and samples from malaria-endemic areas to measure P. vivax-specific IgG responses using different combinations of beads and instruments. We directly compared: i) non-magnetic versus magnetic beads run on a Bio-Plex® 200, ii) magnetic beads run on the Bio-Plex® 200 versus MAGPIX® and iii) non-magnetic beads run on a Bio-Plex® 200 versus magnetic beads run on the MAGPIX®. We also performed an external comparison of our optimized assay. We observed that IgG antibody responses, measured against our panel of P. vivax proteins, were moderately-strongly correlated in all three of our comparisons (pearson r>0.5 for 18/19 proteins), however higher amounts of protein were required for coupling to magnetic beads. Our external comparison indicated that results generated in different laboratories using the same coupled beads are also highly comparable (pearson r>0.7), particularly if a reference standard curve is used.
Competing Interests: I have read the journal's policy and the authors of this manuscript have the following competing interests: RJL, TT and IM are inventors on patent application PCT/US17/67926 on a system, method, apparatus and diagnostic test for Plasmodium vivax. MH is a paid employee of CellFree Sciences Co., Ltd. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
Databáze: MEDLINE
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