Coexpressed subunits of dual genetic origin define a conserved supercomplex mediating essential protein import into chloroplasts.

Autor:	Ramundo S; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143.; Howard Hughes Medical Institute, Chevy Chase, MD 20815., Asakura Y; Laboratory of Organelle Biology, Institute for Protein Research, Osaka University, Osaka 565-0871, Japan., Salomé PA; Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095., Strenkert D; Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095., Boone M; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143.; Howard Hughes Medical Institute, Chevy Chase, MD 20815., Mackinder LCM; Department of Biology, University of York, York YO10 5DD, United Kingdom., Takafuji K; Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan., Dinc E; Department of Molecular Biology, University of Geneva, Geneva CH-1211, Switzerland.; Department of Plant Biology, University of Geneva, Geneva CH-1211, Switzerland., Rahire M; Department of Molecular Biology, University of Geneva, Geneva CH-1211, Switzerland.; Department of Plant Biology, University of Geneva, Geneva CH-1211, Switzerland., Crèvecoeur M; Department of Molecular Biology, University of Geneva, Geneva CH-1211, Switzerland.; Department of Plant Biology, University of Geneva, Geneva CH-1211, Switzerland., Magneschi L; Institute of Plant Biology and Biotechnology, University of Münster, Münster 48143, Germany., Schaad O; Department of Biochemistry, University of Geneva, Geneva CH-1211, Switzerland., Hippler M; Institute of Plant Biology and Biotechnology, University of Münster, Münster 48143, Germany.; Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan., Jonikas MC; Department of Molecular Biology, Princeton University, Princeton, NJ 08540.; Howard Hughes Medical Institute, Chevy Chase, MD 20815., Merchant S; Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095., Nakai M; Laboratory of Organelle Biology, Institute for Protein Research, Osaka University, Osaka 565-0871, Japan; nakai@protein.osaka-u.ac.jp Jean-David.Rochaix@unige.ch peter@walterlab.ucsf.edu., Rochaix JD; Department of Molecular Biology, University of Geneva, Geneva CH-1211, Switzerland; nakai@protein.osaka-u.ac.jp Jean-David.Rochaix@unige.ch peter@walterlab.ucsf.edu.; Department of Plant Biology, University of Geneva, Geneva CH-1211, Switzerland., Walter P; Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143; nakai@protein.osaka-u.ac.jp Jean-David.Rochaix@unige.ch peter@walterlab.ucsf.edu.; Howard Hughes Medical Institute, Chevy Chase, MD 20815.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2020 Dec 22; Vol. 117 (51), pp. 32739-32749. Date of Electronic Publication: 2020 Dec 03.
DOI:	10.1073/pnas.2014294117
Abstrakt:	In photosynthetic eukaryotes, thousands of proteins are translated in the cytosol and imported into the chloroplast through the concerted action of two translocons-termed TOC and TIC-located in the outer and inner membranes of the chloroplast envelope, respectively. The degree to which the molecular composition of the TOC and TIC complexes is conserved over phylogenetic distances has remained controversial. Here, we combine transcriptomic, biochemical, and genetic tools in the green alga Chlamydomonas ( Chlamydomonas reinhardtii ) to demonstrate that, despite a lack of evident sequence conservation for some of its components, the algal TIC complex mirrors the molecular composition of a TIC complex from Arabidopsis thaliana. The Chlamydomonas TIC complex contains three nuclear-encoded subunits, Tic20, Tic56, and Tic100, and one chloroplast-encoded subunit, Tic214, and interacts with the TOC complex, as well as with several uncharacterized proteins to form a stable supercomplex (TIC-TOC), indicating that protein import across both envelope membranes is mechanistically coupled. Expression of the nuclear and chloroplast genes encoding both known and uncharacterized TIC-TOC components is highly coordinated, suggesting that a mechanism for regulating its biogenesis across compartmental boundaries must exist. Conditional repression of Tic214, the only chloroplast-encoded subunit in the TIC-TOC complex, impairs the import of chloroplast proteins with essential roles in chloroplast ribosome biogenesis and protein folding and induces a pleiotropic stress response, including several proteins involved in the chloroplast unfolded protein response. These findings underscore the functional importance of the TIC-TOC supercomplex in maintaining chloroplast proteostasis. Competing Interests: The authors declare no competing interest. (Copyright © 2020 the Author(s). Published by PNAS.)
Databáze:	MEDLINE
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