Molecular detection of extended spectrum β-lactamase genes in Escherichia coli clinical isolates from diarrhoeic children in Kano, Nigeria.

Autor: Saka HK; Nigerian Stored Products Research Institute, Kano, Nigeria.; Department of Microbiology, Bayero University Kano, Kano, Nigeria., García-Soto S; VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain., Dabo NT; Department of Biological Sciences, Bayero University Kano, Kano, Nigeria., Lopez-Chavarrias V; VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain., Muhammad B; Department of Microbiology, Bayero University Kano, Kano, Nigeria., Ugarte-Ruiz M; VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain., Alvarez J; VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain.; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2020 Dec 03; Vol. 15 (12), pp. e0243130. Date of Electronic Publication: 2020 Dec 03 (Print Publication: 2020).
DOI: 10.1371/journal.pone.0243130
Abstrakt: The increase in antimicrobial resistance in developed and developing countries is a global public health challenge. In this context β-lactamase production is a major contributing factor to resistance globally. The aim of this study was to determine the prevalence of phenotypic and genotypic extended spectrum β-lactamases (ESBLs) in 296 E. coli isolates recovered from diarrhoeic children younger than five years in Kano whose susceptibility profile against 7 antimicrobials had been determined. The E. coli isolates were subjected to double disc synergy test for phenotypic ESBLs detection and ESBL associated genes (blaCTX-M, blaTEM and blaSHV) were detected using conventional PCR. Phenotypically, 12.8% (38/296) E. coli isolates presented a ESBLs phenotype, with a significantly higher proportion in isolates from females compared with males (P-value = 0.024). blaCTX-M 73.3% and blaTEM 73.3% were the predominant resistance genes in the ESBLs positive E. coli (each detected in 22/30 isolates, of which 14 harboured both). In addition, 1/30 harboured blaCTX-M + blaTEM + blaSHV genes simultaneously. This study demonstrates the presence of ESBLs E. coli isolates in clinically affected children in Kano, and demonstrates the circulation of blaCTX-M and blaTEM associated with those phenotypes. Enactment of laws on prudent antibiotic use is urgently needed in Kano.
Competing Interests: The authors have declared that no competing interest exist.
Databáze: MEDLINE
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