Transcriptomic profiling of phospholipase A2 and the role of arachidonic acid during Brucella abortus 544 infection in both in vitro and in vivo systems.

Autor: Vu SH; Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea; Institute of Applied Sciences, Ho Chi Minh City University of Technology - HUTECH, 475A Dien Bien Phu St., Ward 25, Binh Thanh District, Ho Chi Minh City, Vietnam, Republic of Korea., Bernardo Reyes AW; Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea., Ngoc Huy TX; Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea., Min W; Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea., Lee HJ; Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea., Kim HJ; Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea., Lee JH; College of Veterinary Medicine, Chonbuk National University, Iksan, 54596, Republic of Korea., Kim S; Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea. Electronic address: kimsuk@gnu.ac.kr.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2021 Mar; Vol. 152, pp. 104655. Date of Electronic Publication: 2020 Nov 29.
DOI: 10.1016/j.micpath.2020.104655
Abstrakt: To date, the antimicrobial activity of arachidonic acid (AA) with regard to pathogenesis of Brucella in macrophages is unknown. We found that AA is highly toxic to B. abortus in a time- and dose-dependent manner. Transcription profiling of different groups of phospholipases A2 (PLA 2 ) was examined, ten PLA 2 were detected including cPLA 2 -IV-A, cPLA 2 -IV-B, iPLA 2 -VI, sPLA 2 -I-B, sPLA 2 -II-C, sPLA 2 -II-D, sPLA 2 -II-E, sPLA 2 -V, sPLA 2 -X, sPLA 2 -XII-A. Phagocytic signaling investigation indicated that AA treatment attenuated p38α activity in infected culture macrophages possibly leading to inhibition of Brucella internalization. Post-treatment with the fatty acid did not influence bacterial intracellular multiplication or alter production of antimicrobial effectors like ROS and NO in RAW 264.7 cells. On the other hand, AA administration significantly reduced bacterial load and modestly inhibited pro-inflammatory cytokine secretion including TNF, IFN-γ and IL-6 in mice plasma. To our knowledge, we are the first to suggest that B. abortus invasion to RAW 264.7 macrophages is impaired by AA.
(Copyright © 2020. Published by Elsevier Ltd.)
Databáze: MEDLINE
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