In Vitro Effects of Lithium Carbonate on Cell Cycle, Apoptosis, and Autophagy in Hepatocellular Carcinoma-29 Cells.

Autor: Taskaeva YS; Research Institute of Clinical and Experimental Lymphology - Affiliated Branch of Federal Research Centre Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. inabrite@yandex.ru.; Novosibirsk National Research State University, Novosibirsk, Russia. inabrite@yandex.ru., Bgatova NP; Research Institute of Clinical and Experimental Lymphology - Affiliated Branch of Federal Research Centre Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia., Dossymbekova RS; Abai Kazakh National Pedagogical University, Almaty, Republic of Kazakhstan., Solovieva AO; Research Institute of Clinical and Experimental Lymphology - Affiliated Branch of Federal Research Centre Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia., Miroshnichenko SM; Research Institute of Clinical and Experimental Lymphology - Affiliated Branch of Federal Research Centre Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.; Research Institute of Biochemistry - Structural Subdivision of Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia., Sharipov KO; S. D. Asfendiyarov Kazakh National Medical University, Almaty, Republic of Kazakhstan., Tungushbaeva ZB; Abai Kazakh National Pedagogical University, Almaty, Republic of Kazakhstan.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2020 Dec; Vol. 170 (2), pp. 246-250. Date of Electronic Publication: 2020 Dec 02.
DOI: 10.1007/s10517-020-05044-9
Abstrakt: We studied the effects of lithium carbonate on the cell cycle, apoptosis, and autophagy in hepatocellular carcinoma-29 cells (HCC-29) in vitro. Flow cytofluorometry analysis revealed accumulation of G2/M-phase HCC-29 cells and increase in the number of apoptotic cells in 48 h after administration of 5 mM lithium carbonate. Induction of autophagy in HCC-29 cells was detected by transmission electron microscopy and immunofluorescence staining. Thus, lithium carbonate produces an antitumor effect by arresting cell cycle in the G2/M-phase and induction of apoptosis and autophagy in HCC-29 cells, which confirms the lithium potential as a promising drug for the treatment of hepatocellular carcinoma.
Databáze: MEDLINE
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