| Autor: |
Gama RS; Universidade Vale do Rio Doce, Laboratório de Biologia Molecular, Governador Valadares, MG, Brasil., Leite LA; Universidade Vale do Rio Doce, Laboratório de Biologia Molecular, Governador Valadares, MG, Brasil., Colombo LT; Universidade Vale do Rio Doce, Laboratório de Biologia Molecular, Governador Valadares, MG, Brasil., Fraga LAO; Universidade Federal de Juiz de Fora, Departamento de Ciências da Vida, Governador Valadares, MG, Brasil. |
| Jazyk: |
angličtina |
| Zdroj: |
Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2020 Nov 25; Vol. 53, pp. e20200197. Date of Electronic Publication: 2020 Nov 25 (Print Publication: 2020). |
| DOI: |
10.1590/0037-8682-0197-2020 |
| Abstrakt: |
Slit skin smear and histopathological examinations are currently the main laboratory tools used to aid the diagnosis of leprosy. However, their sensitivity is low, and many cases are not detected. New methodologies have been studied to develop more accurate tests. This narrative review aims to raise attention to the results of molecular (polymerase chain reaction) and serological (Enzyme-Linked Immunosorbent Assay) tests applied to the diagnosis of leprosy, and to summarize the available information about the former. Original scientific articles published in indexed international journals, whose study involved aspects of the diagnosis and classification of leprosy cases or home contacts, were selected. The data were extracted independently using a standardized method that dictated the inclusion of the following information: diagnosis in Paucibacillary and Multibacillary cases and in household contacts; sample number; sample type; study design; studied variables; statistical analysis employed; main results; and limitations identified. In clinical practice, the results from molecular and serological tests are assessed separately, with moderate sensitivity and specificity. However, an integrated study of these methodologies has been suggested for greater accuracy in diagnosis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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