The effects of collagenase of Clostridium Histolitycum (CCH) in Dupuytren's disease: a histological study on cell mediated mechanisms that allow enzymatic fasciotomy.

Autor:	Passiatore M; Fondazione Policlinico Universitario A. Gemelli IRCCS, Roma, Italia., De Vitis R; Istituto di Clinica Ortopedica, Fondazione Policlinico Universitario A. Gemelli IRCCS, Roma, Italia., Saracco M; Fondazione Policlinico Universitario A. Gemelli IRCCS, Roma, Italia., Brunelli C; Division of Anatomic Pathology and Histology, Fondazione Policlinico Universitario A. Gemelli IRCCS, Roma, Italia., Castri F; Division of Anatomic Pathology and Histology, Fondazione Policlinico Universitario A. Gemelli IRCCS, Roma, Italia., Taccardo G; Istituto di Clinica Ortopedica, Fondazione Policlinico Universitario A. Gemelli IRCCS, Roma, Italia.
Jazyk:	angličtina
Zdroj:	Journal of biological regulators and homeostatic agents [J Biol Regul Homeost Agents] 2020 Jul-Aug; Vol. 34 (4 Suppl. 3), pp. 279-284. Congress of the Italian Orthopaedic Research Society.
Abstrakt:	Dupuytren Disease is a benign fibromatosis of palmar fascia of the hand, whose pathophysiology is not completely understood. The present study is intended to provide a description of the effects of Collagenase of Clostridium Hystoliticum (CCH) into an injected cord of Dupuytren. Our experimental study wanted to evaluate the histological effects of injection of CCH in the first 24 hours, without manipulating the specimens. Materials and Methods: Surgical specimens were injected with CCH, and then fixed in formalin every six hours, up to 24 hours. Those specimens were compared to control specimen (non-injected), fixed and analyzed at the same times. Results: In the injected specimens, the number of CD68 positive cells increased into and outside the cords compared to non injected specimens, within the same time from the surgical removal. Conclusion: CCH has a proinflammatory activity and provokes a short ray chemotactic action on white blood cells. The lysis of the cord induced by CCH stimulates the inflammatory response. The role of the inflammatory infiltration deserves to be investigated in a more accurate way, preferably by using in vivo studies. (Copyright 2020 Biolife Sas. www.biolifesas.org.)
Databáze:	MEDLINE
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