Hydrocarbon-Stapled Peptide Based-Nanoparticles for siRNA Delivery.

Autor:	Simon M; IBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, France., Laroui N; IBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, France., Heyraud M; IBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, France., Laconde G; IBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, France., Ali LMA; IBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, France.; Department of Biochemistry, Medical Research Institute, University of Alexandria, Alexandria 21561, Egypt., Bourbiaux K; IBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, France., Subra G; IBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, France., Vezenkov LL; IBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, France., Legrand B; IBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, France., Amblard M; IBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, France., Bettache N; IBMM, Univ Montpellier, CNRS, ENSCM, 34093 Montpellier, France.
Jazyk:	angličtina
Zdroj:	Nanomaterials (Basel, Switzerland) [Nanomaterials (Basel)] 2020 Nov 25; Vol. 10 (12). Date of Electronic Publication: 2020 Nov 25.
DOI:	10.3390/nano10122334
Abstrakt:	Small interfering RNAs (siRNAs) are promising molecules for developing new therapies based on gene silencing; however, their delivery into cells remains an issue. In this study, we took advantage of stapled peptide technology that has emerged as a valuable strategy to render natural peptides more structured, resistant to protease degradation and more bioavailable, to develop short carriers for siRNA delivery. From the pool of stapled peptides that we have designed and synthesized, we identified non-toxic vectors that were able to efficiently encapsulate siRNA, transport them into the cell and induce gene silencing. Remarkably, the most efficient stapled peptide (JMV6582), is composed of only eight amino-acids and contains only two cationic charges.
Databáze:	MEDLINE
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