Bacterial nanocellulose-hyaluronic acid microneedle patches for skin applications: In vitro and in vivo evaluation.

Autor: Fonseca DFS; CICECO - Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal., Vilela C; CICECO - Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal., Pinto RJB; CICECO - Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal., Bastos V; CESAM, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal., Oliveira H; CESAM, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal., Catarino J; CBIOS - Centre for Research in Biosciences & Health Technologies, Lusófona University of Humanities and Technologies, 1740-024 Lisbon, Portugal., Faísca P; CBIOS - Centre for Research in Biosciences & Health Technologies, Lusófona University of Humanities and Technologies, 1740-024 Lisbon, Portugal; Faculty of Veterinary Medicine, Lusófona University of Humanities and Technologies, Lisbon, Portugal., Rosado C; CBIOS - Centre for Research in Biosciences & Health Technologies, Lusófona University of Humanities and Technologies, 1740-024 Lisbon, Portugal., Silvestre AJD; CICECO - Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal., Freire CSR; CICECO - Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address: cfreire@ua.pt.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2021 Jan; Vol. 118, pp. 111350. Date of Electronic Publication: 2020 Aug 11.
DOI: 10.1016/j.msec.2020.111350
Abstrakt: The aim of the present study was to develop innovative patches for dermo-cosmetic applications based on dissolvable hyaluronic acid (HA) microneedles (MNs) combined with bacterial nanocellulose (BC) as the back layer. HA was employed as an active biomacromolecule, with hydrating and regenerative properties and volumizing effect, whereas BC was used as support for the incorporation of an additional bioactive molecule. Rutin, a natural antioxidant, was selected as the model bioactive compound to demonstrate the effectiveness of the system. The obtained HA-MNs arrays present homogenous and regular needles, with 200 μm in base width, 450 μm in height and 500 μm tip-to-tip distance, and with sufficient mechanical force to withstand skin insertion with a failure force higher than 0.15 N per needle. The antioxidant activity of rutin was neither affected by its incorporation in the MNs system nor by their storage at room temperature for 6 months. Preliminary in vivo studies in human volunteers unveiled their safety and cutaneous compatibility, as no significant changes in barrier function, stratum corneum hydration nor redness were detected. These results confirm the potentiality of this novel system for skin applications, e.g. cosmetics, taking advantage of the recognized properties of HA and the capacity of BC to control the release of bioactive molecules.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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