| Autor: |
Wang H; Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, Germany.; Chair for Molecular Nutritional Medicine, Technical University of Munich, Freising, Germany., Willershäuser M; Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, Germany.; Chair for Molecular Nutritional Medicine, Technical University of Munich, Freising, Germany., Li Y; Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, Germany.; Chair for Molecular Nutritional Medicine, Technical University of Munich, Freising, Germany., Fromme T; Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, Germany.; Chair for Molecular Nutritional Medicine, Technical University of Munich, Freising, Germany., Schnabl K; Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, Germany.; Chair for Molecular Nutritional Medicine, Technical University of Munich, Freising, Germany., Bast-Habersbrunner A; Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, Germany.; Chair for Molecular Nutritional Medicine, Technical University of Munich, Freising, Germany., Ramisch S; Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, Germany.; Chair for Molecular Nutritional Medicine, Technical University of Munich, Freising, Germany., Mocek S; Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, Germany.; Chair for Molecular Nutritional Medicine, Technical University of Munich, Freising, Germany., Klingenspor M; Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, Germany.; Chair for Molecular Nutritional Medicine, Technical University of Munich, Freising, Germany. |
| Abstrakt: |
We studied the metabolic phenotype of a novel Ucp1 -LUC-iRFP713 knock-in reporter gene mouse model originally generated to monitor endogenous Ucp1 gene expression. Both reporter mice and reporter cells reliably reflected Ucp1 gene expression in vivo and in vitro. We here report an unexpected reduction in UCP1 content in homozygous knock-in (KI) reporter mice. As a result, the thermogenic capacity of KI mice stimulated by norepinephrine was largely blunted, making them more sensitive to an acute cold exposure. In return, these reporter mice with reduced UCP1 expression enabled us to investigate the physiological role of UCP1 in the prevention of weight gain. We observed no substantial differences in body mass across the three genotypes, irrespective of the type of diet or the ambient temperature, possibly due to the insufficient UCP1 activation. Indeed, activation of UCP1 by daily injection of the selective β 3 -adrenergic receptor agonist CL316,243 resulted in significantly greater reduction of body weight in wild-type mice than in KI mice. Taken together, we conclude that the intact expression of UCP1 is essential for cold-induced thermogenesis but the presence of UCP1 per se does not protect mice from diet-induced obesity. NEW & NOTEWORTHY To study the functional role of UCP1-dependent brown adipose tissue thermogenesis for energy balance, new animal models are needed. By metabolic phenotyping of a novel mouse model with low UCP1 levels in brown fat, we demonstrate that the susceptibility to diet-induced obesity is not increased despite impaired cold-induced thermogenic capacity. Brown fat requires pharmacological activation to promote negative energy balance in diet-induced obese mice. |
