Melatonin improves periodontitis-induced kidney damage by decreasing inflammatory stress and apoptosis in rats.

Autor: Kose O; Department of Periodontology, School of Dentistry, Recep Tayyip Erdogan University, Rize, Turkey., Kurt Bayrakdar S; Department of Periodontology, School of Dentistry, Eskişehir Osmangazi University, Eskisehir, Turkey., Unver B; Department of Periodontology, School of Dentistry, Recep Tayyip Erdogan University, Rize, Turkey., Altin A; Department of Periodontology, School of Dentistry, Recep Tayyip Erdogan University, Rize, Turkey., Akyildiz K; Department of Medical Services and Techniques, School of Healh Care Services Vocational, Recep Tayyip Erdogan University, Rize, Turkey., Mercantepe T; Department of Histology and Embryology, School of Medicine, Recep Tayyip Erdogan University, Rize, Turkey., Bostan SA; Department of Periodontology, School of Dentistry, Recep Tayyip Erdogan University, Rize, Turkey., Arabaci T; Department of Periodontology, School of Dentistry, Ataturk University, Erzurum, Turkey., Turker Sener L; Department of Biophysics School of Medicine, Istanbul University, Istanbul, Turkey., Emre Kose T; Department of Dentomaxillofacial Radiology, School of Dentistry, Recep Tayyip Erdogan University, Rize, Turkey., Tumkaya L; Department of Histology and Embryology, School of Medicine, Recep Tayyip Erdogan University, Rize, Turkey., Yilmaz A; Department of Biochemistry, School of Medicine, Recep Tayyip Erdogan University, Rize, Turkey., Kuluslu G; 3D Medical and Industrial Design Laboratory, Istanbul University, Istanbul, Turkey.
Jazyk: angličtina
Zdroj: Journal of periodontology [J Periodontol] 2021 Jun; Vol. 92 (6), pp. 22-34. Date of Electronic Publication: 2020 Nov 30.
DOI: 10.1002/JPER.20-0434
Abstrakt: Background: Two main aims of this animal study were to inspect the possible effects of periodontitis on the structure and functions of the kidneys and the therapeutic effectiveness of melatonin.
Methods: Twenty-four male Sprague-Dawley rats were randomly divided into three groups: control, experimental periodontitis (Ep), and Ep-melatonin (Ep-Mel). Periodontitis was induced by placing 3.0-silk sutures sub-paramarginally around the cervix of right-left mandibular first molars and maintaining the sutures for 5 weeks. Then melatonin (10 mg/kg body weight/day, 14 days), and the vehicle was administered intraperitonally. Mandibular and kidney tissue samples were obtained following the euthanasia. Periodontal bone loss was measured via histological and microcomputed tomographic slices. On right kidney histopathological and immunohistochemical, and on the left kidney biochemical (malonyl-aldehyde [MDA], glutathione, oxidative stress [OSI], tumor necrosis factor [TNF]-α, interleukin [IL]-1β, matrix metalloproteinase [MMP]-8, MMP-9, and cathepsin D levels) evaluations were performed. Renal functional status was analyzed by levels of serum creatinine, urea, cystatin-C, and urea creatinine.
Results: Melatonin significantly restricted ligature-induced periodontal bone loss (P <0 .01) and suppressed the levels of proinflammatory cytokines (TNF-α and IL-1β), oxidative stress (MDA and OSI), and proteases (MMP-8, MMP-9, and CtD) that was significantly higher in the kidneys of the rats with periodontitis (P <0.05). In addition, periodontitis-related histological damages and apoptotic activity were also significantly lower in the Ep-Mel group (P <0.05). However, the markers of renal function of the Ep group were detected slightly impaired in comparison with the control group (P >0.05); and the therapeutic activity of melatonin was limited (P >0.05).
Conclusion: Melatonin restricts the periodontitis-induced inflammatory stress, apoptosis, and structural but not functional impairments.
(© 2020 American Academy of Periodontology.)
Databáze: MEDLINE
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