Virulence, resistance and clonality of Proteus mirabilis isolated from patients with community-acquired urinary tract infection (CA-UTI) in Brazil.

Autor: Danilo de Oliveira W; Laboratory of Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina (UEL), Londrina, PR, Brazil., Lopes Barboza MG; Laboratory of Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina (UEL), Londrina, PR, Brazil., Faustino G; Laboratory of Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina (UEL), Londrina, PR, Brazil., Yamanaka Inagaki WT; Laboratory of Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina (UEL), Londrina, PR, Brazil., Sanches MS; Laboratory of Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina (UEL), Londrina, PR, Brazil., Takayama Kobayashi RK; Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina (UEL), Londrina, PR, Brazil., Vespero EC; Department of Pathology and Clinical and Toxicological Analysis, State University of Londrina (UEL), Londrina, PR, Brazil., Dejato Rocha SP; Laboratory of Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina (UEL), Londrina, PR, Brazil. Electronic address: rochaspd@uel.br.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2021 Mar; Vol. 152, pp. 104642. Date of Electronic Publication: 2020 Nov 25.
DOI: 10.1016/j.micpath.2020.104642
Abstrakt: Urinary tract infections (UTIs) are among the most common human infections, both in hospitals and in communities. Proteus mirabilis is known to cause community-acquired urinary tract infection (CA-UTI) and is an important causative agent of nosocomial UTIs. The pathogenesis of this species is related to its ability to manifest virulence factors, such as biofilms, adhesion molecules, urease, proteases, siderophores, and toxins. In this study, we investigated the virulence, sensitivity to antimicrobials, and clonal relationship of 183 strains isolated from the urine of CA-UTI patients in Londrina, Paraná State, Brazil. A total of 100% of the strains were positive for hpmA, ptA, zapA, mrpA, pmfA, ireA, and atfA virulence genes. The ucaA gene was positive in 81.4% of the cases. The strains showed high rates of sensitivity to the evaluated antimicrobials, and only one was ESBL-positive. All the tested bacteria showed the capacity to form biofilms: 73.2% had a very strong intensity, while 25.7% had a strong intensity, and 1.1% had a moderate intensity. Regarding clonality, 40 clonal clusters were found among the microorganisms tested. Our results showed that strains of P. mirabilis isolated from CA-UTI patients have several virulence factors. Although the urinary clinical isolates studied showed high sensitivity to antimicrobials, the strains showed a strong capacity to form biofilms, making antibiotic therapy difficult. In addition, it was observed that there were clones of P. mirabilis circulating in the city of Londrina.
(Copyright © 2020. Published by Elsevier Ltd.)
Databáze: MEDLINE
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