A new method for isolating and analysing coccospheres within sediment.

Autor: Langley B; Geography, College of Life and Environmental Sciences, University of Exeter, Exeter, EX4 4RJ, UK., Halloran PR; Geography, College of Life and Environmental Sciences, University of Exeter, Exeter, EX4 4RJ, UK. p.halloran@exeter.ac.uk., Power A; Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter, EX4 4QD, UK., Rickaby REM; Department of Earth Sciences, University of Oxford, South Parks Road, Oxford, OX1 3AN, UK., Chana P; Luminex B.V., Het Zuiderkruis 1, 5215 MV, 's-Hertogenbosch, The Netherlands., Diver P; Department of Earth Sciences, University of Oxford, South Parks Road, Oxford, OX1 3AN, UK., Thornalley D; Department of Geography, University College London, London, WC1H 9LG, UK., Hacker C; Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter, EX4 4QD, UK., Love J; Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter, EX4 4QD, UK.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2020 Nov 26; Vol. 10 (1), pp. 20727. Date of Electronic Publication: 2020 Nov 26.
DOI: 10.1038/s41598-020-77473-5
Abstrakt: Size is a fundamental cellular trait that is important in determining phytoplankton physiological and ecological processes. Fossil coccospheres, the external calcite structure produced by the excretion of interlocking plates by the phytoplankton coccolithophores, can provide a rare window into cell size in the past. Coccospheres are delicate however and are therefore poorly preserved in sediment. We demonstrate a novel technique combining imaging flow cytometry and cross-polarised light (ISX +PL ) to rapidly and reliably visually isolate and quantify the morphological characteristics of coccospheres from marine sediment by exploiting their unique optical and morphological properties. Imaging flow cytometry combines the morphological information provided by microscopy with high sample numbers associated with flow cytometry. High throughput imaging overcomes the constraints of labour-intensive manual microscopy and allows statistically robust analysis of morphological features and coccosphere concentration despite low coccosphere concentrations in sediments. Applying this technique to the fine-fraction of sediments, hundreds of coccospheres can be visually isolated quickly with minimal sample preparation. This approach has the potential to enable rapid processing of down-core sediment records and/or high spatial coverage from surface sediments and may prove valuable in investigating the interplay between climate change and coccolithophore physiological/ecological response.
Databáze: MEDLINE
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