Abl and Canoe/Afadin mediate mechanotransduction at tricellular junctions.

Autor: Yu HH; Howard Hughes Medical Institute and Developmental Biology Program, Sloan Kettering Institute, New York, NY, USA., Zallen JA; Howard Hughes Medical Institute and Developmental Biology Program, Sloan Kettering Institute, New York, NY, USA. zallenj@mskcc.org.
Jazyk: angličtina
Zdroj: Science (New York, N.Y.) [Science] 2020 Nov 27; Vol. 370 (6520).
DOI: 10.1126/science.aba5528
Abstrakt: Epithelial structure is generated by the dynamic reorganization of cells in response to mechanical forces. Adherens junctions transmit forces between cells, but how cells sense and respond to these forces in vivo is not well understood. We identify a mechanotransduction pathway involving the Abl tyrosine kinase and Canoe/Afadin that stabilizes cell adhesion under tension at tricellular junctions in the Drosophila embryo. Canoe is recruited to tricellular junctions in response to actomyosin contractility, and this mechanosensitivity requires Abl-dependent phosphorylation of a conserved tyrosine in the Canoe actin-binding domain. Preventing Canoe tyrosine phosphorylation destabilizes tricellular adhesion, and anchoring Canoe at tricellular junctions independently of mechanical inputs aberrantly stabilizes adhesion, arresting cell rearrangement. These results identify a force-responsive mechanism that stabilizes tricellular adhesion under tension during epithelial remodeling.
(Copyright © 2020, American Association for the Advancement of Science.)
Databáze: MEDLINE
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