Rapid Method for the Determination of Thiamine and Pantothenic Acid in Infant Formula and Milk-Based Nutritional Products by Liquid Chromatography‒Tandem Mass Spectrometry.
| Autor: | Gill BD; Fonterra Co-operative Group Ltd, P.O. Box 7, Waitoa, 3341, New Zealand., Saldo SC; Fonterra Co-operative Group Ltd, P.O. Box 7, Waitoa, 3341, New Zealand., McGrail IJ; Fonterra Co-operative Group Ltd, P.O. Box 7, Waitoa, 3341, New Zealand., Wood JE; Fonterra Co-operative Group Ltd, P.O. Box 7, Waitoa, 3341, New Zealand., Indyk HE; Fonterra Co-operative Group Ltd, P.O. Box 7, Waitoa, 3341, New Zealand. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of AOAC International [J AOAC Int] 2020 Jun 01; Vol. 103 (3), pp. 812-817. |
| DOI: | 10.1093/jaoacint/qsz034 |
| Abstrakt: | Background: Thiamine and pantothenic acid play a critical role in numerous metabolic reactions and are typically supplemented in infant and adult nutritional formulas as thiamine chloride hydrochloride and calcium pantothenate salts. Objective: A rapid compliance method for the analysis of thiamine and pantothenic acid applicable to infant formula and milk-based nutritional products is described. Method: Proteins are removed by centrifugal ultrafiltration, followed by analysis by reversed-phase liquid chromatography‒tandem mass spectrometry (LC-MS/MS), with quantitation accomplished by internal standard technique. Results: The method was shown to be accurate, with acceptable recovery (thiamine, 99.3-101.1%; pantothenic acid, 99.2-108.6%). A certified reference material (NIST 1849a), showed no statistical bias (α = 0.05) for thiamine (P = 0.64); although a statistically significant bias (P < 0.01) for pantothenic acid was found, the nominal bias was only 4.7% (mean = 7.1 mg/hg; certified value = 6.8 mg/hg). A comparison of results by LC-MS/MS and current methods showed negligible bias (mean bias: thiamine, 0.01 mg/hg; pantothenic acid, 0.17 mg/hg) and no statistical significance (α = 0.05; thiamine, P = 0.399; pantothenic acid, P = 0.058). Acceptable precision was demonstrated with a repeatability of 7.2% repeatability relative standard deviation (RSDr) (HorRat: 0.6) and an intermediate precision of 7.0% RSD for thiamine, and a repeatability of 5.7% RSDr (HorRat: 0.5) and an intermediate precision of 6.1% RSD for pantothenic acid. Conclusions: This rapid method is intended for use in high-throughput laboratories as part of routine product compliance release testing of thiamine and pantothenic acid in manufactured infant and milk-based nutritional products. (© AOAC INTERNATIONAL 2020. All rights reserved. For permissions, please email: journals.permissions@oup.com.) |
| Databáze: | MEDLINE |
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