| Autor: |
Miller BS; London Centre for Nanotechnology, University College London, London, UK. ben.miller.13@ucl.ac.uk.; Division of Medicine, University College London, London, UK. ben.miller.13@ucl.ac.uk., Bezinge L; London Centre for Nanotechnology, University College London, London, UK., Gliddon HD; London Centre for Nanotechnology, University College London, London, UK., Huang D; London Centre for Nanotechnology, University College London, London, UK., Dold G; London Centre for Nanotechnology, University College London, London, UK.; Department of Electronic and Electrical Engineering, University College London, London, UK., Gray ER; London Centre for Nanotechnology, University College London, London, UK., Heaney J; Advanced Pathogens Diagnostic Unit, University College London Hospitals, London, UK., Dobson PJ; The Queens College, University of Oxford, Oxford, UK., Nastouli E; Department of Virology, University College London Hospitals, London, UK., Morton JJL; London Centre for Nanotechnology, University College London, London, UK.; Department of Electronic and Electrical Engineering, University College London, London, UK., McKendry RA; London Centre for Nanotechnology, University College London, London, UK. r.a.mckendry@ucl.ac.uk.; Division of Medicine, University College London, London, UK. r.a.mckendry@ucl.ac.uk. |
| Abstrakt: |
The quantum spin properties of nitrogen-vacancy defects in diamond enable diverse applications in quantum computing and communications 1 . However, fluorescent nanodiamonds also have attractive properties for in vitro biosensing, including brightness 2 , low cost 3 and selective manipulation of their emission 4 . Nanoparticle-based biosensors are essential for the early detection of disease, but they often lack the required sensitivity. Here we investigate fluorescent nanodiamonds as an ultrasensitive label for in vitro diagnostics, using a microwave field to modulate emission intensity 5 and frequency-domain analysis 6 to separate the signal from background autofluorescence 7 , which typically limits sensitivity. Focusing on the widely used, low-cost lateral flow format as an exemplar, we achieve a detection limit of 8.2 × 10 -19 molar for a biotin-avidin model, 10 5 times more sensitive than that obtained using gold nanoparticles. Single-copy detection of HIV-1 RNA can be achieved with the addition of a 10-minute isothermal amplification step, and is further demonstrated using a clinical plasma sample with an extraction step. This ultrasensitive quantum diagnostics platform is applicable to numerous diagnostic test formats and diseases, and has the potential to transform early diagnosis of disease for the benefit of patients and populations. |
Full text from SpringerLink