Phenotypic and genotypic characterization of non-typhoidal Salmonella isolated from a Brazilian pork production chain.

Autor: Viana C; Universidade Federal de Viçosa, Departamento de Veterinária, Laboratório de Inspeção de Produtos de Origem Animal - InsPOA, Avenida PH Rolfs, s/n, Campus Universitário, 36570-900 Viçosa, MG, Brazil., Grossi JL; Universidade Federal de Viçosa, Departamento de Veterinária, Laboratório de Inspeção de Produtos de Origem Animal - InsPOA, Avenida PH Rolfs, s/n, Campus Universitário, 36570-900 Viçosa, MG, Brazil., Sereno MJ; Universidade Federal de Viçosa, Departamento de Veterinária, Laboratório de Inspeção de Produtos de Origem Animal - InsPOA, Avenida PH Rolfs, s/n, Campus Universitário, 36570-900 Viçosa, MG, Brazil., Yamatogi RS; Universidade Federal de Viçosa, Departamento de Veterinária, Laboratório de Inspeção de Produtos de Origem Animal - InsPOA, Avenida PH Rolfs, s/n, Campus Universitário, 36570-900 Viçosa, MG, Brazil., Bersot LDS; Universidade Federal do Paraná - Setor Palotina, Departamento de Ciências Veterinárias, Laboratório de Inspeção e Controle de Qualidade de Alimentos e Água - LACOMA, Rua Pioneiro, 2153, Jardim Dallas, 85950-000 Palotina, PR, Brazil., Call DR; Washington State University, Paul G. Allen School for Global Animal Health, 240 SE Ott Road PO Box 647090, 99164-7090 Pullman, WA, USA., Nero LA; Universidade Federal de Viçosa, Departamento de Veterinária, Laboratório de Inspeção de Produtos de Origem Animal - InsPOA, Avenida PH Rolfs, s/n, Campus Universitário, 36570-900 Viçosa, MG, Brazil. Electronic address: nero@ufv.br.
Jazyk: angličtina
Zdroj: Food research international (Ottawa, Ont.) [Food Res Int] 2020 Nov; Vol. 137, pp. 109406. Date of Electronic Publication: 2020 Jun 08.
DOI: 10.1016/j.foodres.2020.109406
Abstrakt: Pork products are important sources of foodborne non-typhoidal Salmonella in Brazil where antibiotics are commonly used throughout the pork production process and this has the potential to selectively favor antibiotic-resistant strains. We characterized the genotypic and phenotypic diversity of S. enterica isolates (n = 41) that were isolated in Brazil. Isolates were collected from ten swine farms and one slaughterhouse. Whole-genome sequencing and in silico serotyping demonstrated that the S. enterica serovar Typhimurium was the most common serotype (n = 17), but eight additional servoars were identified. Isolates presented high similarity based on comparison of DNA sequences (minimum of 89.6%), and sequence variation grouped according to serotype. Eight multilocus sequence types were identified with ST19 being most common (n = 21). Several plasmids replicons were detected, with Col (RNAI) the most abundant (n = 30), followed by IncR (n = 22), IncI1 (n = 10) and IncA/C2 (n = 10). Minimum inhibitory concentration assays showed that the principle resistance phenotypes were for streptomycin (90.2%), tetracycline (87.8%), ampicillin (80.5%), chloramphenicol (70.7%) and ciprofloxacin (51.2%). Only two isolates were resistant to third-generation cephalosporins and no isolates were resistant to two tested carbapenems. Twenty-six unique antimicrobial-resistance genes were identified with bla TEM-1A and bla TEM-1B likely responsible for most beta-lactam resistance and floR responsible for most chloramphenicol resistance. Six strains were positive for mcr-1. At the time of collection, the sampled farms were adding ciprofloxacin to feed and this may have contributed to the high prevalence of resistance to this antibiotic. The high number of multidrug resistant Salmonella and the presence of multiple resistant genes and plasmids emphasize the diversity of Salmonella in the studied pork chain, specially from serotype Typhimurium.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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