Discriminating lineages of Batrachochytrium dendrobatidis using quantitative PCR.

Autor: Ghosh PN; MRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London, UK.; Unit for Environmental Sciences and Management, North-West University, Potchefstroom, South Africa., Verster R; Unit for Environmental Sciences and Management, North-West University, Potchefstroom, South Africa., Sewell TR; MRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London, UK., O'Hanlon SJ; MRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London, UK., Brookes LM; MRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London, UK.; Institute of Zoology, Zoological Society of London, London, UK.; The Royal Veterinary College, Hatfield, UK., Rieux A; CIRAD, UMR, PVBMT, St Pierre, La Réunion, France., Garner TWJ; Unit for Environmental Sciences and Management, North-West University, Potchefstroom, South Africa.; Institute of Zoology, Zoological Society of London, London, UK., Weldon C; Unit for Environmental Sciences and Management, North-West University, Potchefstroom, South Africa., Fisher MC; MRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London, UK.
Jazyk: angličtina
Zdroj: Molecular ecology resources [Mol Ecol Resour] 2021 Jul; Vol. 21 (5), pp. 1452-1459. Date of Electronic Publication: 2021 Feb 12.
DOI: 10.1111/1755-0998.13299
Abstrakt: The ability to detect and monitor infectious disease in a phylogenetically informative manner is critical for their management. Phylogenetically informative diagnostic tests enable patterns of pathogen introduction or changes in the distribution of genotypes to be measured, enabling research into the ecology of the pathogen. Batrachochytrium dendrobatidis (Bd), a causative agent of chytridiomycosis in amphibian populations, emerged worldwide in the 21st century and is composed of six lineages which are display varying levels of virulence in their hosts. Research into the distribution, ecology and pathogenicity of these lineages has been hampered by an inability to type lineage efficiently. Here, we describe a lineage-specific TaqMan qPCR assay that differentiates the two lineages of Bd most commonly associated with chytridiomycosis: BdGPL and BdCAPE. We demonstrate how this assay can be used for the surveillance of wild populations of amphibians in Southern Africa using skin swabs, tissue samples and cultured isolates.
(© 2020 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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