Deep phenotypical characterization of human CD3 + CD56 + T cells by mass cytometry.

Autor: Romero-Olmedo AJ; Institute for Medical Microbiology and Hospital Hygiene, University of Marburg, Marburg, Germany., Schulz AR; German Rheumatism Research Center Berlin (DRFZ), Leibniz Institute, Berlin, Germany., Huber M; Institute for Medical Microbiology and Hospital Hygiene, University of Marburg, Marburg, Germany., Brehm CU; Comprehensive Biobank Marburg - CBBMR, Member of the DZL, Philipps-University Marburg, Marburg, Germany.; Institute for Pathology, University Hospital Marburg, Philipps-University Marburg, Marburg, Germany., Chang HD; German Rheumatism Research Center Berlin (DRFZ), Leibniz Institute, Berlin, Germany., Chiarolla CM; Institute of Pathology, Julius-Maximilian University of Wuerzburg, Wuerzburg, Germany., Bopp T; Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany., Skevaki C; Institute of Laboratory Medicine, Universities of Giessen and Marburg Lung Center (UGMLC), Philipps University Marburg, German Center for Lung Research (DZL), Marburg, Germany., Berberich-Siebelt F; Institute of Pathology, Julius-Maximilian University of Wuerzburg, Wuerzburg, Germany., Radbruch A; German Rheumatism Research Center Berlin (DRFZ), Leibniz Institute, Berlin, Germany., Mei HE; German Rheumatism Research Center Berlin (DRFZ), Leibniz Institute, Berlin, Germany., Lohoff M; Institute for Medical Microbiology and Hospital Hygiene, University of Marburg, Marburg, Germany.
Jazyk: angličtina
Zdroj: European journal of immunology [Eur J Immunol] 2021 Mar; Vol. 51 (3), pp. 672-681. Date of Electronic Publication: 2020 Dec 15.
DOI: 10.1002/eji.202048941
Abstrakt: CD56 + T cells are a group of pro-inflammatory CD3 + lymphocytes with characteristics of natural killer cells, being involved in antimicrobial immune defense. Here, we performed deep phenotypic profiling of CD3 + CD56 + cells in peripheral blood of normal human donors and individuals sensitized to birch-pollen or/and house dust mite by high-dimensional mass cytometry combined with manual and computational data analysis. A co-regulation between major conventional T-cell subsets and their respective CD3 + CD56 + cell counterparts appeared restricted to CD8 + , MAIT, and TCRγδ + T-cell compartments. Interestingly, we find a co-regulation of several CD3 + CD56 + cell subsets in allergic but not in healthy individuals. Moreover, using FlowSOM, we distinguished a variety of CD56 + T-cell phenotypes demonstrating a hitherto underestimated heterogeneity among these cells. The novel CD3 + CD56 + subset description comprises phenotypes superimposed with naive, memory, type 1, 2, and 17 differentiation stages, in part represented by a phenotypical continuum. Frequencies of two out of 19 CD3 + CD56 + FlowSOM clusters were significantly diminished in allergic individuals, demonstrating less frequent presence of cells with cytolytic, presumably protective, capacity in these donors consistent with defective expansion or their recruitment to the affected tissue. Our results contribute to defining specific cell populations to be targeted during therapy for allergic conditions.
(© 2020 The Authors. European Journal of Immunology published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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