| Autor: |
Olack BJ; Integrated Islet Distribution Program, Department of Diabetes & Cancer Discovery Science, City of Hope, Duarte, CA, USA., Alexander M; Department of Surgery, University of California Irvine, Orange, CA, USA., Swanson CJ; Integrated Islet Distribution Program, Department of Diabetes & Cancer Discovery Science, City of Hope, Duarte, CA, USA., Kilburn J; Integrated Islet Distribution Program, Department of Diabetes & Cancer Discovery Science, City of Hope, Duarte, CA, USA., Corrales N; Department of Surgery, University of California Irvine, Orange, CA, USA., Flores A; Department of Surgery, University of California Irvine, Orange, CA, USA., Heng J; Department of Surgery, University of California Irvine, Orange, CA, USA., Arulmoli J; Scharp-Lacy Research Institute, Aliso Viejo, CA, USA., Omori K; Department of Translational Research and Cellular Therapeutics, City of Hope, Duarte, CA, USA., Chlebeck PJ; Department of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA., Zitur L; Department of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA., Salgado M; Department of Translational Research and Cellular Therapeutics, City of Hope, Duarte, CA, USA., Lakey JRT; Department of Surgery, University of California Irvine, Orange, CA, USA.; Department of Biomedical Engineering, University of California Irvine, Irvine, CA, USA., Niland JC; Integrated Islet Distribution Program, Department of Diabetes & Cancer Discovery Science, City of Hope, Duarte, CA, USA. |
| Abstrakt: |
Access to functional high-quality pancreatic human islets is critical to advance diabetes research. The Integrated Islet Distribution Program (IIDP), a major source for human islet distribution for over 15 years, conducted a study to evaluate the most advantageous times to ship islets postisolation to maximize islet recovery. For the evaluation, three experienced IIDP Islet Isolation Centers each provided samples from five human islet isolations, shipping 10,000 islet equivalents (IEQ) at four different time periods postislet isolation (no 37°C culture and shipped within 0 to 18 hours; or held in 37°C culture for 18 to 42, 48 to 96, or 144 to 192 hours). A central evaluation center compared samples for islet quantity, quality, and viability for each experimental condition preshipment and postshipment, as well as post 37°C culture 18 to 24 hours after shipment receipt. Additional evaluations included measures of functional potency by static glucose-stimulated insulin release (GSIR), represented as a stimulation index. Comparing the results of the four preshipment holding periods, the greatest IEQ loss postshipment occurred with the shortest preshipment times. Similar patterns emerged when comparing preshipment to postculture losses. In vitro islet function (GSIR) was not adversely impacted by increased tissue culture time. These data indicate that allowing time for islet recovery postisolation, prior to shipping, yields less islet loss during shipment without decreasing islet function. |
