| Autor: |
Zakharova EV; All-Russia Research Institute of Agricultural Biotechnology, Moscow, Russia. zakharova_ekater@mail.ru., Timofeeva GV; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia., Fateev AD; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia., Kovaleva LV; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia. |
| Jazyk: |
angličtina |
| Zdroj: |
Protoplasma [Protoplasma] 2021 May; Vol. 258 (3), pp. 573-586. Date of Electronic Publication: 2020 Nov 24. |
| DOI: |
10.1007/s00709-020-01587-w |
| Abstrakt: |
S-RNAse-based self-incompatibility (SI) in petunia (Petunia hybrida L.) is a self-/non-self-recognition system underlying the pistil rejection of self-pollen. Using different methods, including a TUNEL assay, we have recently shown that programmed cell death (PCD) is a factor of the SI in petunia. Here, we show that the growth of self-incompatible pollen tubes in the style tissues during 4 h after pollination is accompanied by five-sixfold increase in a caspase-like protease (CLP) activity. Exogenous cytokinin (CK) inhibits the pollen tube growth and stimulates the CLP activity in compatible pollen tubes. The actin depolymerization with latrunculin B induces a sharp drop in the CLP activity in self-incompatible pollen tubes and its increase in compatible pollen tubes. Altogether, our results suggest that a CLP is involved in the SI-induced PCD and that CK is a putative activator of the CLP. We assume that CK provokes acidification of the cytosol and thus promotes the activation of a CLP. Thus, our results suggest that CK and CLP are involved in the S-RNAse-based SI-induced PCD in petunia. Potential relations between these components in PCD signaling are discussed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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