Association of nad7a Gene with Cytoplasmic Male Sterility in Pigeonpea.

Autor: Sinha P; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, 502324, India., Saxena KB; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, 502324, India., Saxena RK; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, 502324, India., Singh VK; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, 502324, India., Suryanarayana V; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, 502324, India., Sameer Kumar CV; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, 502324, India., Katta MAVS; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, 502324, India., Khan AW; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, 502324, India., Varshney RK; International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, 502324, India.; School of Plant Biology and Institute of Agriculture, The Univ. of Western Australia, 35 Stirling Hwy., Crawley, WA, 6009, Australia.
Jazyk: angličtina
Zdroj: The plant genome [Plant Genome] 2015 Jul; Vol. 8 (2), pp. eplantgenome2014.11.0084.
DOI: 10.3835/plantgenome2014.11.0084
Abstrakt: Cytoplasmic male sterility (CMS) has been exploited in the commercial pigeonpea [Cajanus cajan (L.) Millsp.] hybrid breeding system; however, the molecular mechanism behind this system is unknown. To understand the underlying molecular mechanism involved in A 4 CMS system derived from C. cajanifolius (Haines) Maesen, 34 mitochondrial genes were analyzed for expression profiling and structural variation analysis between CMS line (ICRISAT Pigeonpea A line, ICPA 2039) and its cognate maintainer (ICPB 2039). Expression profiling of 34 mitochondrial genes revealed nine genes with significant fold differential gene expression at P ≤ 0.01, including one gene, nad4L, with 1366-fold higher expression in CMS line as compared with the maintainer. Structural variation analysis of these mitochondrial genes identified length variation between ICPA 2039 and ICPB 2039 for nad7a (subunit of nad7 gene). Sanger sequencing of nad4L and nad7a genes in the CMS and the maintainer lines identified two single nucleotide polymorphisms (SNPs) in upstream region of nad4L and a deletion of 10 bp in nad7a in the CMS line. Protein structure evaluation showed conformational changes in predicted protein structures for nad7a between ICPA 2039 and ICPB 2039 lines. All above analyses indicate association of nad7a gene with the CMS for A 4 cytoplasm in pigeonpea. Additionally, one polymerase chain reaction (PCR) based Indel marker (nad7a_del) has been developed and validated for testing genetic purity of A 4 derived CMS lines to strengthen the commercial hybrid breeding program in pigeonpea.
(© 2015 The Authors.)
Databáze: MEDLINE
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